Tissue distribution of monochloroacetic acid and its binding to albumin in rats.
Kaphalia-BS; Bhat-HK; Khan-MF; Ansari-GA
Toxicol Ind Health 1992 Jan; 8(1-2):53-61
The distribution patterns of monochloroacetic-acid (79118) (CA) were examined in rats. Male Sprague-Dawley-rats were administered a single oral dose of carbon-14 labeled CA at 0.1 millimole/kilogram by gavage. Rats were then placed in metabolic cages, and urine was collected at 4, 8, 12, 24, and 48 hours after dosing. Selected rats were sacrificed at each time point, and organs were analyzed for CA distribution. Rats in another group were treated with 1 millimole/kilogram CA daily by gavage for 3 days, and sacrificed 24 hours after the last dose. Results indicated that CA was rapidly absorbed and excreted from the body. The elimination phase appeared to be fast for intestine and kidney as compared to other tissues. Maximum radioactivity was detected in intestine and kidney at 4 and 8 hours after treatment; in decreasing order, radioactivity was also detected in liver, spleen, testes, lung, brain and heart. Examination of carbon-14 labeling in dialyzed plasma suggested that CA bound to plasma proteins in-vivo; albumin accounted for about 65% of the accumulation as determined by affinity chromatography. The authors suggest that isolation and identification of the adducts of amino acids will assist in understanding the mechanism of CA toxicity and the development of a suitable biomarker of CA exposure.
NIOSH-Publication; NIOSH-Grant; Grants-other; Laboratory-animals; Tissue-distribution; Chlorinated-hydrocarbons; Metabolic-study; Urinalysis; Protein-chemistry
Human Biol Chem and Genetics University of Texas Med BR Dept of Human Biol Chem&gene Galveston, Tex 77550-2774
Other Occupational Concerns; Grants-other
Toxicology and Industrial Health
University of Texas Medical Branch, Galveston, Texas