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Nonideal behavior of alkoxyphenoxazone compounds (cytochrome P-450 substrates) in aqueous solution.

Authors
Rabovsky-J; Heflin-E; Judy-DJ; Sapola-NA
Source
J Biochem Toxicol 1989 Summer; 4(2):127-132
NIOSHTIC No.
00205405
Abstract
The spectral properties of alkoxyphenoxazone compounds in organic and aqueous solutions were studied. Solutions of methoxyphenoxazone (MeOPx), ethoxyphenoxazone, pentoxyphenoxazone (PeOPx) and benzyloxyphenoxazone, ranging from 0.4 millimolar (mM) to 2mM were prepared in 2% methylsulfoxide. Spectral properties were measured in absolute ethanol (EtOH) or an aqueous solvent (HEPES). In EtOH, each compound exhibited a peak at 460 nanometers (nm) and a shoulder at 390nm and the Lambert/Beer law was obeyed. In HEPES, only MeOPx obeyed the Lambert/Beer law; peak absorption spectra for the other three compounds were shifted to lower wavelengths and the extinction coefficients decreased with increasing concentrations. These changes were dependent on the side chains. The blue shift and extinction coefficients seen at high PeOPx concentrations in HEPES changed with temperature. Resorufin and phenoxazone obeyed the Lambert/Beer law in both EtOH and HEPES. The authors conclude that spectral data obtained from aqueous solutions of alkoxyphenoxazone compounds may be unreliable.
Keywords
NIOSH-Author; Aromatic-hydrocarbons; Analytical-methods; Chemical-properties; Spectrographic-analysis; Aqueous-solutions; Chemical-structure; Analytical-chemistry; Author Keywords: Alkoxyphenoxazone; Cytochrome P-450; Spectral Properties
CODEN
JBTOEB
Publication Date
19890601
Document Type
Journal Article
Fiscal Year
1989
NTIS Accession No.
NTIS Price
Issue of Publication
2
ISSN
0887-2082
NIOSH Division
DRDS
Source Name
Journal of Biochemical Toxicology
State
WV
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