Hydrocarbon solvents: effect on calcium mediated events in liposomes.
Rabovsky-J; Marion-KJ; Resnick-H
Chem Phys Lipids 1985 Apr; 37(1):13-18
Experiments were conducted to determine the effects of hydrocarbon solvents on the calcium 2+ ion (Ca2+) induced turbidity in liposomes. Anionic liposomes consisting of a phospholipid bilayer containing dimyristoylphosphatidylcholine and 4% molar dicetylphosphate were prepared and the turbidity measured at 360 nanometers after 16 to 18 hours of incubation with test substances. Ca2+ had a maximal effect on the liposomes at concentrations between increase in turbidity. A reversal of Ca2+ induced turbidity of liposomes was seen with exposure to carbon-tetrachloride (56235) (CCl4), 1,1,1-trichloroethane (71556) (TCE), chloroform (67663) (CHCl3), cyclohexane (110827), hexane (110543) and benzene (71432). The data conformed to double reciprocal plot analysis. Apparent association constants were determined to be: 0.11mM(-1) for CCl4, 1) with 1mm Ca2+ for TCE. Based on these results the authors suggest that halogenated aliphatic solvents have a weak interaction with membrane binding sites.
NIOSH-Author; Organic-solvents; Halogenated-hydrocarbons; Chemical-kinetics; Chemical-properties; Phospholipids; Plasma-membrane; Chemical-analysis;
Author Keywords: liposomes; calcium; solvents
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Chemistry and Physics of Lipids