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Triphenyl phosphite-induced ultrastructural changes in bovine adrenomedullary chromaffin cells.

Authors
Knoth-Anderson-J; Veronesi-B; Jones-K; Lapadula-DM; Abou-Donia-MB
Source
Toxicol Appl Pharmacol 1992 Jan; 112(1):110-119
NIOSHTIC No.
00205254
Abstract
Transmission electron microscopy (TEM) and scanning electron microscopy (TEM) were used to assess the morphological changes in primary cultures of bovine adrenomedullary chromaffin cells treated with triphenyl-phosphite (101020) (TPP). The cells were isolated and cultured with 100 micromolar (microM) TPP, O,O- diisopropylphosphorofluoridate (55914) (DFP), paraoxon (311455), or vehicle for 24 or 1 hour prior to fixing and TEM examination. For SEM, the cells were similarly treated for 24 hours. Radiolabeled adenosine incorporation into ATP was assessed through 0.25, 0.5, 1, 4, or 24 hour treatments with 100microM of the chemicals and thin layer chromatography. After 24 hours, almost all mitochondria were disrupted or swollen; glycogen buildup was evident. SEM revealed low viability for TPP treated cells. Filopodia were contracted, surface texture was irregular, and giant globular bodies were present. Paraoxon and DFP did not cause the same ultrastructural effects. The authors conclude that TPP's effect is specific and that its target is the mitochondria.
Keywords
NIOSH-Publication; NIOSH-Grant; Neurotoxic-effects; Organo-phosphorus-compounds; Cell-cultures; Cytotoxic-effects; Nucleosides; Cell-morphology; Microscopic-analysis; Biochemical-tests;
Contact
Pharmacology Duke University Department of Pharmacology Durham, N C 27710
CODEN
TXAPA9
CAS No.
101-02-0; 55-91-4; 311-45-5
Publication Date
19920101
Document Type
Journal Article
Funding Amount
1567389
Funding Type
Grant
Fiscal Year
1992
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-00823
Issue of Publication
1
ISSN
0041-008X
Priority Area
Neurotoxic Disorders; Neurotoxic-effects
Source Name
Toxicology and Applied Pharmacology
State
NC
Performing Organization
Duke University, Durham, North Carolina
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