Micronuclei assay in cytokinesis-blocked binucleated and conventional mononucleated methods in human peripheral lymphocytes.
Teratog, Carcinog, Mutagen 1990 Jan; 10(3):273-279
The effect of cytochalasin-B (14930962) (CYB) on the induction of chromosomal aberrations, and the frequencies of micronuclei induced by treatment of cells with mitomycin-C (50077) (MMC) and cyclophosphamide (50180) (CPA), were examined. Lymphocytes from a normal individual were isolated, cultured for 48 hours and exposed to 1, 2, 3, 4, 5.5, 7.5, or 10 micrograms/milliliter (microg/ml) for 24 hours. Certain cultures were also exposed to MMC after 40 hours of incubation, or incubated with CPA for 3 hours, washed and incubated with 3microg/ml CYB for an additional 24 hours. Cells were harvested, fixed, stained and examined for micronuclei. A CYB dose dependent increase in micronuclei was seen, with peak numbers of binucleated and multinucleated cells occurring with doses of 3 to 4microg/ml CYB. Both MMC and CPA treatment resulted in dose dependent increases in micronuclei frequencies when tested on the CYB cytokinesis blocked binucleated cells and on conventional mononuclear cells.
NIOSH-Author; Cell-cultures; Cell-division; Chromosome-damage; Toxic-effects; Dose-response; Analytical-methods; Laboratory-techniques;
Author Keywords: micronucleus assay; human lymphocytes; cytochalasin-B; binucleated cell; mononucleated cell
T. Ong, National Institute for Occupational Safety and Health, Division of Respiratory Disease Studies, 944 Chestnut Ridge Road, Morgantown, West Virginia 26505-2888
14930-96-2; 50-07-7; 50-18-0
Teratogenesis, Carcinogenesis, and Mutagenesis