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Micronuclei assay in cytokinesis-blocked binucleated and conventional mononucleated methods in human peripheral lymphocytes.

Authors
Channarayappa-JNa; Ong-T
Source
Teratog, Carcinog, Mutagen 1990 Jan; 10(3):273-279
NIOSHTIC No.
00204966
Abstract
The effect of cytochalasin-B (14930962) (CYB) on the induction of chromosomal aberrations, and the frequencies of micronuclei induced by treatment of cells with mitomycin-C (50077) (MMC) and cyclophosphamide (50180) (CPA), were examined. Lymphocytes from a normal individual were isolated, cultured for 48 hours and exposed to 1, 2, 3, 4, 5.5, 7.5, or 10 micrograms/milliliter (microg/ml) for 24 hours. Certain cultures were also exposed to MMC after 40 hours of incubation, or incubated with CPA for 3 hours, washed and incubated with 3microg/ml CYB for an additional 24 hours. Cells were harvested, fixed, stained and examined for micronuclei. A CYB dose dependent increase in micronuclei was seen, with peak numbers of binucleated and multinucleated cells occurring with doses of 3 to 4microg/ml CYB. Both MMC and CPA treatment resulted in dose dependent increases in micronuclei frequencies when tested on the CYB cytokinesis blocked binucleated cells and on conventional mononuclear cells.
Keywords
NIOSH-Author; Cell-cultures; Cell-division; Chromosome-damage; Toxic-effects; Dose-response; Analytical-methods; Laboratory-techniques; Author Keywords: micronucleus assay; human lymphocytes; cytochalasin-B; binucleated cell; mononucleated cell
Contact
T. Ong, National Institute for Occupational Safety and Health, Division of Respiratory Disease Studies, 944 Chestnut Ridge Road, Morgantown, West Virginia 26505-2888
CODEN
TCMUD8
CAS No.
14930-96-2; 50-07-7; 50-18-0
Publication Date
19900101
Document Type
Journal Article
Fiscal Year
1990
NTIS Accession No.
NTIS Price
Issue of Publication
3
ISSN
0270-3211
NIOSH Division
DRDS
Source Name
Teratogenesis, Carcinogenesis, and Mutagenesis
State
WV
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