Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Evaluation of Drosophila for screening developmental toxicants: test results with eighteen chemicals and presentation of a new Drosophila bioassay.

Authors
Lynch-DW; Schuler-RL; Hood-RD; Davis-RG
Source
Teratog, Carcinog, Mutagen 1991Jan; 11(3):147-173
NIOSHTIC No.
00203730
Abstract
A modified Drosophila assay for screening developmental toxicants was described. The assay utilized Oregon-R wild type Drosophila- melanogaster. Flies were exposed to the test agent from the egg through three larval stages by incorporating five range finding concentrations of the chemical into the culture medium. A total of 200 emergent adult flies were examined intact and scored for a bent humeral bristle and a notch in the wing blade. After the data were analyzed by probit techniques, the concentrations of the test agent were adjusted to provide at least three challenge concentrations. The protocol was repeated using a minimum of 500 pooled flies per concentration over three runs. If statistically significant results were not obtained, additional trials using adjusted concentrations were performed. The modified assay was used to evaluate 18 compounds. Urethane (51796), meprobamate (57534), hydroxyurea (127071), vincristine-sulfate (2068782), procarbazine-hydrochloride (366701), 2,4,5-T (93765), caffeine (58082), acetazolamide (59665), EM-12 (26581817), 6-aminonicotineamide (329895), acetylsalicylic- acid (50782), ethanol (64175), and cyclophosphamide (50180) were known developmental toxicants. Sodium-saccharin (128449), formaldehyde (50000), diphenylhydramine-hydrochloride (147240), nitrilotriacetic-acid (139139), and amaranth (915673) were nondevelopmental toxicants. Among the developmental toxicants, all except ethanol, acetylsalicylic-acid, and cyclophosphamide gave positive responses in the modified assay. All nondevelopmental toxicants except sodium-saccharin were identified correctly. The responses indicated a sensitivity of the modified assay of 77%, a specificity of 80%, and overall accuracy of 78%. Experiments leading to optimization of the assay were described. The authors conclude that the modified assay using intact Drosophila can be used to screen chemicals for developmental toxicity.
Keywords
NIOSH-Author; In-vivo-studies; Insects; Bioassays; Teratogens; Laboratory-techniques; Organic-compounds; Screening-methods
Contact
Dennis W. Lynch, National Institute for Occupational Safety and Health (Mail Stop C-23), Robert A. Taft Laboratories, 4676 Columbia Parkway, Cincinnati, OH 45226-1998
CODEN
TCMUD8
CAS No.
51-79-6; 57-53-4; 127-07-1; 2068-78-2; 366-70-1; 93-76-5; 58-08-2; 59-66-5; 26581-81-7; 329-89-5; 50-78-2; 64-17-5; 50-18-0; 128-44-9; 50-00-0; 147-24-0; 139-13-9; 915-67-3
Publication Date
19910101
Document Type
Journal Article
Fiscal Year
1991
NTIS Accession No.
NTIS Price
Issue of Publication
3
ISSN
0270-3211
NIOSH Division
DBBS
Source Name
Teratogenesis, Carcinogenesis, and Mutagenesis
State
OR; OH
TOP