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Peripheral markers of muscarinic receptors.

Authors
Costa-LG
Source
Department of Environmental Health, University of Washington, Seattle, Washington 1990 Jul; :1-7
Link
NIOSHTIC No.
00203288
Abstract
These studies were designed to provide information regarding the biochemical and functional characteristics of muscarinic receptors on lymphocytes and on the possibility of the use of these receptors as markers of muscarinic receptors in tissues not accessible in humans. Such knowledge will assist in assessing the effect of exposure of humans to chemicals which affect the nervous system. An extensive characterization of muscarinic binding sites in rat lymphocytes was performed. A series of eight muscarinic antagonists and five agonists were tested for their ability to inhibit tritium labeled QNB binding to lymphocytes. The authors conclude that a binding site for tritium labeled QNB is present on rat splenic and circulating lymphocytes which has most of the pharmacological characteristics of muscarininc cholinergic receptors. The major difference appears to be a lower affinity of muscarinic antagonists between lymphocytes and the brain. The muscarinic receptors would be tentatively of the M2 type as suggested by the high affinity of oxotremorine, the low affinity of pirenzepine, the strong effect of GTP on agonist binding and the lack of stimulation of phosphoinositide metabolism by cholinergic agonist.
Keywords
NIOSH-Grant; Grants-other; Nervous-system-disorders; Neurotoxins; Laboratory-animals; Biological-monitoring
Contact
Environmental Health University of Washington Dept of Environ Hlth, SC-34 Seattle, WA 98195
Publication Date
19900726
Document Type
Final Grant Report
Funding Amount
97100
Funding Type
Grant
Fiscal Year
1990
NTIS Accession No.
PB92-135961
NTIS Price
A02
Identifying No.
Grant-Number-K01-OH-00054
NIOSH Division
OEP
Priority Area
Other Occupational Concerns; Grants-other
Source Name
Department of Environmental Health, University of Washington, Seattle, Washington
State
WA
Performing Organization
University of Washington, Seattle, Washington
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