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Analysis of urine to monitor exposures to denzidine, o-dianisidine, o-tolidine, and 4,4'-methylenedianiline.

Authors
Neumeister-CE
Source
Appl Occup Environ Hyg 1991 Nov; 6(11):953-958
NIOSHTIC No.
00203126
Abstract
A sampling and analytical procedure was developed for measuring urine concentrations of benzidine (92875), ortho-dianisidine (119904), ortho-tolidine (119937), and 4,4'-methylenedianiline (101779) for use in industrial hygiene investigations. After hydrolysis of urine metabolites, the free diamines were isolated using a C18 solid sorbent. The free diamines were eluted, concentrated, and injected into a high performance liquid chromatography system. Using a C18 column with a mobile phase of acetonitrile and water, separation was achieved. By monitoring the ultraviolet absorbance at 280 or 245 nanometers and the electrochemical response, identification and quantitation were achieved. The limit of detection for these aromatic diamines was less than 2 micrograms/liter using ultraviolet detection while the limit of quantitation was less than 6 micrograms/liter. For electrochemical detection the limit of detection was less than 0.3 micrograms/liter and the limit of quantitation was less than 0.9 micrograms/liter. Recoveries ranged from 87 to 102% at 2, 10, and 20 micrograms/liter levels.
Keywords
NIOSH-Author; Urinalysis; Anilines; Benzidines; Toluidines; Biological-monitoring; Quantitative-analysis; Analytical-chemistry; Analytical-methods; Chemical-analysis
CODEN
AOEHE9
CAS No.
92-87-5; 119-90-4; 119-93-7; 101-77-9;
Publication Date
19911101
Document Type
Journal Article
Fiscal Year
1992
NTIS Accession No.
NTIS Price
Issue of Publication
11
ISSN
1047-322X
Source Name
Applied Occupational and Environmental Hygiene
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