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Immunologic methods for the detection of benzo(a)pyrene metabolites in urine.

Authors
Gomes-M; Santella-RM
Source
Chem Res Toxicol 1990 Jul; 3(4):307-310
NIOSHTIC No.
00202990
Abstract
Monoclonal antibodies were developed for the detection of benzo(a)pyrene (50328) (BaP) and its metabolites in urine. Spleen cells from Balb/C-mice immunized with BaP linked to bovine serum albumin were isolated and fused with myeloma cell line P3x63- AG.8.653. Antibodies were purified and named 4D5 and 10C10. An immunoaffinity column of antibody 4D5 was made to concentrate BaP from urine. A competitive enzyme linked immunosorbent assay (ELISA) was used for quantitative measurement of BaP and metabolites. At four picomole BaP, there was a 50% inhibition with both antibodies. The antibodies also cross reacted with the metabolites of BaP and with other polyaromatic hydrocarbons (PAH). Mice were injected with tritium labeled BaP and urine was collected to determine if these antibodies could be used with biological samples. Analysis with 4D5 gave 50% inhibition at 12 to 15 picomole BaP metabolite. The authors conclude that this assay is useful as a general indicator of exposure though the antibodies cross react with other PAH so quantitation is difficult.
Keywords
NIOSH-Publication; NIOSH-Grant; Dermatitis; Immunochemistry; Polycyclic-aromatic-hydrocarbons; Immunological-tests; Immunodiagnosis; In-vitro-studies; Urine-chemistry; Analytical-methods
Contact
Environmental Sciences Columbia University 650 West 168Th Street New York, NY 10032
CODEN
CRTOEC
CAS No.
50-32-8
Publication Date
19900701
Document Type
Journal Article
Funding Amount
651727
Funding Type
Grant
Fiscal Year
1990
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-002622
Issue of Publication
4
ISSN
0893-228X
Priority Area
Dermatitis
Source Name
Chemical Research in Toxicology
State
NY
Performing Organization
Columbia University New York, New York, New York
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