Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Effect of ammonium metavanadate on the mouse peritoneal macrophage lysosomal enzymes.

Authors
Vaddi-K; Wei-C
Source
J Toxicol Environ Health 1991 May; 33(1):65-78
NIOSHTIC No.
00200973
Abstract
The effect of vanadate on the activities and kinetics of select lysosomal enzymes was examined. The possibility that this was a contributing mechanism to the depression of the intracellular killing ability in peritoneal macrophages (PEM) from vanadate treated mice was considered. Ammonium-metavanadate (7803556) at 2.5 or 10mg/kg vanadium (7440622), ammonium-chloride (12125029), or sodium-phosphate buffer were used to inject intraperitoneally female B6C3F1-mice every third day for 6 weeks. Acid-phosphatase activity for the 2.5 and 10mg/kg vanadium groups was depressed by 22.8 and 44%, respectively. Vanadium treatment did not cause any significant effect on the other three enzymes. Ammonium-metavanadate caused similar effects as vanadium. Ammonium-chloride and vanadium treatments caused an enhancement of extracellular secretion of beta- glucuronidase and lysozyme from PEM. This could be attributed to the presence of ammonium ion. The findings suggested that the decreased bactericidal activity in the PEM of vanadate treated mice was not due to direct inhibition of either activity or synthesis of beta-glucuronidase, N-acetyl-beta-D-glucosaminidase or lysozyme. The authors suggest that the decrease in acid-phosphatase activity may contribute to the diminished intracellular killing ability of PEM.
Keywords
NIOSH-Publication; NIOSH-Grant; Enzyme-activity; Cytotoxic-effects; Laboratory-animals; Cell-damage; Vanadium-compounds; Cell-function; Chemical-kinetics
Contact
Food Science & Human Nutrition University of Florida Gainesville, FL 32611 Gainesville, FL 32611
CODEN
JTEHD6
CAS No.
7803-55-6; 7440-62-2; 12125-02-9
Publication Date
19910501
Document Type
Journal Article
Funding Amount
43500.00
Funding Type
Grant
Fiscal Year
1991
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R03-OH-02583
Issue of Publication
1
ISSN
0098-4108
Source Name
Journal of Toxicology and Environmental Health
State
FL
Performing Organization
University of Florida, Gainesville, Florida
TOP