Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Light and electron microscopic evidence of tri-o-cresyl phosphate (TOCP)-mediated testicular toxicity in Fischer 344 rats.

Authors
Somkuti-SG; Lapadula-DM; Chapin-RE; Abou-Donia-MB
Source
Toxicol Appl Pharmacol 1991 Jan; 107(1):35-46
NIOSHTIC No.
00198820
Abstract
A time course study was done in rats to examine testicular toxicity of tri-o-cresyl-phosphate (78308) (TOCP) by light and electron microscopy. Male Fischer-344-rats were given 150mg/kg TOCP in corn- oil by gavage for 1, 3, 5, 7, 10, 14 or 21 successive days. Sections of testis and epididymis were prepared and analyzed by light and transmission electron microscopy. TOCP did not significantly alter body weights of treated rats, and no clinical signs of organophosphate toxicity were observed. Lesions were first noted after 3 days of exposure and indicated inhibition of spermiation and of condensation of spermatid residual bodies. By 5 days, columnar and spherical shaped vacuoles were noted in stage-XII tubule germinal epithelium and were localized by electron microscopy to Sertoli cells. Widespread dilation of smooth and rough endoplasmic reticulum was noted, along with basally located spermatid heads in Sertoli cells. Severity of lesions increased with time. Primary changes included increased seminiferous epithelial vacuolation and numbers of abnormal residual bodies and giant cells, along with sperm karyorrhexis. Sperm density per tubule decreased with continued exposure such that 90% of seminiferous tubules were without sperm by 14 days. In later stages, spermatid or spermatocyte nuclei were observed within multinucleated giant cells. Spermatogonia did not appear to be affected, and Leydig cells demonstrated no consistent alterations. The authors conclude that TOCP has an initial toxic effect on Sertoli cells and subsequently causes severe disruption of spermatogenesis.
Keywords
NIOSH-Publication; NIOSH-Grant; Neurotoxic-effects; Laboratory-animals; In-vivo-studies; Toxic-effects; Organo-phosphorus-compounds; Reproductive-system; Microscopic-analysis; Histopathology
Contact
Pharmacology Duke University Medical Center Box 3813 Durham, NC 27710
CODEN
TXAPA9
CAS No.
78-30-8
Publication Date
19910101
Document Type
Journal Article
Funding Amount
665055
Funding Type
Grant
Fiscal Year
1991
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-02003
Issue of Publication
1
ISSN
0041-008X
Priority Area
Neurotoxic Disorders; Neurotoxic-effects
Source Name
Toxicology and Applied Pharmacology
State
NC
Performing Organization
Duke University, Durham, North Carolina
TOP