Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Alterations in Pulmonary Xenobiotic Metabolizing Enzyme Systems in Asbestotic Animals.

Authors
Rahman-Q; Khan-SG; Ali-S
Source
NIOSH 1990 Nov:1330-1335
NIOSHTIC No.
00198796
Abstract
Changes in pulmonary xenobiotic metabolizing systems induced by asbestos (1332214) were studied in rats. Male albino rats were administered 0 or 5 milligrams chrysotile (12001295) dust intratracheally. Selected rats were killed 1, 4, 8, 16, 90, or 290 days later and the lungs were removed and weighed. Lung cytosol and microsome fractions were prepared. Microsomal cytochrome-P-450 (P450) and glutathione content and benzo(a)pyrene-hydroxylase (BaPOHase) and epoxide-hydratase (EH) activity were determined. The extent of microsomal lipid peroxidation was measured. Cytosolic ascorbic-acid content and glutathione-S-transferase (GST) activity were determined. Lung weights were significantly increased in chrysotile treated rats examined after 90 and 290 days. Chrysotile caused significant decreases in P450 content and BaPOHase and EH activity 1 to 16 days after exposure. The decreases were followed by significant increases at days 90 and 290. GST activity and glutathione content decreased progressively with time, the decreases becoming significant after 90 days. Ascorbic-acid content was significantly decreased 90 and 290 days after chrysotile. Microsomal lipid peroxidation was significantly increased at all time points. The authors conclude that asbestos fibers alter the pulmonary mixed function oxidase system in rats. These changes result in carcinogenic reactive intermediates that are ineffectively cleared being generated, preformed conjugates being hydrolyzed, free radicals being generated, and antioxidants concentrations being decreased. Such changes may favor induction of bronchogenic carcinoma.
Keywords
In-vivo-studies; Laboratory-animals; Asbestos-fibers; Physiological-chemistry; Lung-tissue; Enzyme-complexes; Biotransformation; Lung-cancer; Lipid-peroxidation;
CAS No.
1332-21-4; 12001-29-5;
Publication Date
19901101
Document Type
Conference/Symposia Proceedings;
Fiscal Year
1991
NTIS Accession No.
NTIS Price
Identifying No.
(NIOSH) 90-108
Source Name
Proceedings of the VIIth International Pneumoconioses Conference
State
PA;
TOP