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Biological safety cabinets: considerations for the industrial hygienist.

Authors
Bloom-TF; Osorio-AM
Source
Appl Occup Environ Hyg 1991 Feb; 6(2):119-124
NIOSHTIC No.
00198064
Abstract
An investigation was undertaken of the primary containment capability of biological safety cabinets (BSCs) in connection with an exposure to Legionella-pneumophila at a research laboratory. Major factors of concern included cabinet selection, cabinet certification, cabinet user information and cabinet documents. A visual check of the external cabinet structure and of the cabinet workspace was performed. Information regarding prescribed cabinet uses and limitations was obtained from the manufacturer. This cabinet exhausted filtered air into the testing room atmosphere. However, the filter test data indicated that small amounts of unfiltered air may have been intermittently released to the room atmosphere. Exhaust of this air may have resulted in the uptaking of L-pneumophila. Uptake could also have occurred as a result of leakage of contaminated air through defects found in the cabinet structure. The industrial hygiene manager or investigator must be aware that the type of BSC for a given application depends on the specific agent being manipulated and the degree of protection required. Once the choice is made and the cabinet installed, it must be maintained and tested at scheduled intervals. Personnel must be instructed in the proper usage of the cabinet as well as any limitations.
Keywords
NIOSH-Author; Laboratory-workers; Safety-equipment; Air-quality-monitoring; Bacterial-disease; Safety-practices; Disease-prevention
CODEN
AOEHE9
Publication Date
19910201
Document Type
Journal Article
Fiscal Year
1991
NTIS Accession No.
NTIS Price
Issue of Publication
2
ISSN
1047-322X
Source Name
Applied Occupational and Environmental Hygiene
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