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Inhibition of metabolic coupling by metals.

Authors
Loch-Caruso-R; Corcos-IA; Trosko-JE
Source
J Toxicol Environ Health 1991 Jan; 32(1):33-48
NIOSHTIC No.
00197790
Abstract
This study examined whether certain metal compounds could alter junctional communication. Metabolic coupling was used to assay cell to cell communication in cultured Chinese-hamster-V79 cells. Wild type Chinese-hamster-V79 cells proficient in metabolism of 6- thioguanine (6-TG) were cocultured with mutant V79 cells unable to metabolize 6-TG to its toxic metabolite (resistant cells). Inhibition of metabolic coupling in the presence of 6-TG by the metals was demonstrated as an increase in the recovery of 6-TG resistant cells. Significantly increased recoveries of 6-TG resistant cells were demonstrated by the toxic metal compounds arsenic(V)-acid (1327522), mercurous-chloride (7546307), lead(II)- acetate (15347576), and nickel(II)-chloride (7718549) at concentrations which did not change cell survival. Since the increased recovery with nickel showed no dependence on concentration, its significance may be negligible. Cadmium-chloride (10108642) increased 6-TG resistant cell recovery, but only at a toxic concentration. Zinc-sulfate (7733020) did not increase recovery at all. This cellular response to arsenic (7440382), mercury (7439976), and lead (7439921), the inhibition of junction mediated intercellular communication, has not been previously reported.
Keywords
NIOSH-Publication; NIOSH-Grant; Reproductive-system-disorders; Cell-cultures; Cytotoxic-effects; Mercury-poisoning; Mercury-compounds; Lead-poisoning; Lead-compounds; Cadmium-compounds; Arsenic-compounds; Arsenic-poisoning; Metal-poisoning
Contact
Environmental & Indust Health University of Michigan 109 Observatory Street Ann Arbor, MI 48109-2029
CODEN
JTEHD6
CAS No.
1327-52-2; 7546-30-7; 15347-57-6; 7718-54-9; 10108-64-2; 7733-02-0; 7440-38-2; 7439-97-6; 7439-92-1
Publication Date
19910101
Document Type
Journal Article
Funding Amount
50228.00
Funding Type
Grant
Fiscal Year
1991
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-K01-OH-00059
Issue of Publication
1
ISSN
0098-4108
Priority Area
Reproductive-system-disorders
Source Name
Journal of Toxicology and Environmental Health
State
MI
Performing Organization
University of Michigan at Ann Arbor, Ann Arbor, Michigan
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