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Platelet-activating factor-induced aggregation of rat alveolar macrophages.

Authors
Rabovsky-J; Goddard-M; Pailes-WH; Judy-DJ; Castranova-V
Source
Res Commun Chem Pathol Pharmacol 1990 Aug; 69(2):163-172
Link
NIOSHTIC No.
00197566
Abstract
The aggregation of rat alveolar macrophages in the presence of platelet activating factor (PAF) is described. Male Sprague-Dawley- rats were used in these studies. Addition of PAF to a suspension of rat alveolar macrophages resulted in a rapid change in light transmitted through this cell suspension. The extent of PAF induced aggregation, calculated as a change in transmitted light per 1,000,000 cells per milliliter was 22.9 at a PAF concentration of 94.5 micromolar. Aggregation increased in a linear fashion with increasing cell number up to approximately 3,000,000 cells/milliliter. At cell concentrations greater than this, the extent of aggregation declined. Saturation of the aggregation effect was observed after 80 micromolar PAF concentrations were reached. The authors suggest that the PAF concentration required for half maximal response was similar for rat lung alveolar macrophages and type-II cells, whereas the extent of response of macrophages to PAF was only 66% of that elicited by type-II cells. The stereoisomer, enantio-PAF, and the C2-acetyl hydrolyzed product, lyso-PAF, each caused aggregation of isolated rat alveolar macrophages in a manner similar to that for PAF. The authors suggest that the response of rat macrophages described represents a toxic response to high levels of PAF.
Keywords
NIOSH-Author; Alveolar-cells; Cell-damage; Toxic-effects; Cell-function; Platelets; Lung-cells; Phospholipids
CODEN
RCOCB8
Publication Date
19900801
Document Type
Journal Article
Fiscal Year
1990
NTIS Accession No.
NTIS Price
Issue of Publication
2
ISSN
0034-5164
NIOSH Division
DRDS
Source Name
Research Communications in Chemical Pathology and Pharmacology
State
WV
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