Defense mechanisms of alveolar pneumocytes against occupational agents.
The purposes of this study were to develop and test methods to monitor cytokine production by isolated pneumocytes, to determine if this cellular product exhibits interferon like properties, to determine the time course of interferon production and its dependence on pneumocyte count and viral concentration in type II cells and alveolar macrophages, to compare the magnitude of interferon production by the two types of pneumocytes, and to characterize this interferon. Type II pneumocytes and alveolar macrophages were isolated from the lungs of male Sprague-Dawley-rats and cultured in the presence of inactivated influenza virus. The results indicated that both alveolar macrophages and type II cells produce a cytokine with interferon like properties after exposure to influenza virus. Maximal interferon production was 16 to 20 hours after viral induction. The data suggest that interferon production per cell is higher in type II cells than alveolar macrophages. The relative ability of the cytokine produced by rat pneumocytes to protect guinea-pig cells suggested that the cytokine was, at least in part, the alpha-interferon form.
NIOSH-Author; Laboratory-animals; Alveolar-cells; Cell-damage; Protein-chemistry; Antigens; Lung-cells; Viral-infections; In-vitro-study; Immune-reaction
Division of Respiratory Disease Studies, NIOSH, U.S. Department of Health and Human Services, 31 pages, 18 references