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Effects of trifluoroacetic acid, a halothane metabolite, on C6 glioma cells.

Authors
Ma-TG; Ling-YH; McClure-GD; Tseng-MT
Source
J Toxicol Environ Health 1990 Oct; 31(2):147-158
NIOSHTIC No.
00197019
Abstract
An investigation was conducted to determine the impact of trifluoroacetic-acid (76051) (TFA) on the growth, protein synthesis, and glycosylation of a murine glioma cell, C6, in-vitro. One day exposure to TFA caused a slight concentration dependent enhancement of cell growth. At 8.38 millimolar (mM) a 28% increase was noted. Exposure for 1 to 5 days caused an increase in leucine incorporation into C6 cells. The high dose of TFA (10mM) suppressed the intracellular ATP, GTP, and CTP pool. At lower concentrations, TFA elevated ATP levels 11 to 15% while it exerted no influence on UTP or CTP. Intracellular GTP concentration declined in a dose dependent fashion. One day exposure at 5mM reduced the rate of mannose incorporation into glycoproteins and dolichol linked oligosaccharide to 71 and 39% of control levels, respectively. Five days exposure caused further decline in mannose incorporation and caused an additional effect on the dolichol linked oligosaccharides. No significant decline in glucosamine incorporation in glycoprotein and dolichol linked oligosaccharide was observed. Treatment for 24 hours with 5 and 15mM TFA accelerated lectin induced agglutination. The observed loss of terminal sialyl residues in TFA treated cells could cause an increase in the amount of terminally exposed galactose residues. Such surfaces bind more readily to RCA, and the cells thus become more sensitive to the treatment of this particular lectin.
Keywords
NIOSH-Publication; NIOSH-Grant; Protein-synthesis; Protein-chemistry; Cell-cultures; Fluorinated-hydrocarbons; Anesthetics; Metabolites
Contact
Anatomy University of Louisville Anatomy and Neurobiology Louisville, KY 40292
CODEN
JTEHD6
CAS No.
76-05-1
Publication Date
19901001
Document Type
Journal Article
Funding Amount
43589.00
Funding Type
Grant
Fiscal Year
1991
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R03-OH-02578
Issue of Publication
2
ISSN
0098-4108
Source Name
Journal of Toxicology and Environmental Health
State
KY
Performing Organization
University of Louisville, Louisville, Kentucky
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