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In vitro fertilization of mouse ova by spermatozoa exposed isothermally to radio-frequency radiation.

Authors
Cleary-SF; Liu-LM; Graham-R; East-J
Source
Bioelectromagnetics 1989; 10(4):361-369
NIOSHTIC No.
00191688
Abstract
Mouse spermatozoa were exposed isothermally to radio frequency (RF) electromagnetic radiation and tested for ability to fertilize mouse ova in-vitro. Continuous wave RF radiation at 27 or 2450 megahertz (MHz) was administered for 1 hour to solutions of 10(3) to 10(4) motile sperm per milliliter, at 37 degrees-C. Sperm were prepared from CD1-breeder-mice and ova were obtained from F(1)(C57B1/6 X C3H)- mice. Gametes were coincubated at 37 degrees-C for 4.5 hours, ova were washed free of adherent sperm, fixed and examined by phase contrast microscopy for evidence of fertilization. Exposure to 27MHz radiation at the specific absorption rate (SAR) of 50 or 90 watts/kilogram (W/kg) caused significant reductions in fertilization of 50 and 27 percent. For 2450MHz RF radiation, reductions in fertilization of 38 and 52 percent were observed for 50 and 80W/kg, respectively. Electron microscopy revealed no apparent effects of RF radiation on sperm morphology, ultrastructure, or capacitation. The authors conclude that the significant reduction of in-vitro fertilization rates observed when spermatozoa are exposed to 27 or 2450MHz continuous wave radiation at SARs of 50W/kg or greater is not due to an increase in temperature, nor to a decrease in sperm motility. The applicability of the data to human RF exposure was discussed.
Keywords
NIOSH-Publication; NIOSH-Grant; Electromagnetic-radiation; Radiation-exposure; In-vitro-studies; Fertility; Reproductive-effects; Laboratory-animals; Reproductive-system-disorders; Radio-waves; Radiation-hazards
Contact
Physiology and Biophysics Medical College of Virginia Box 694, Mcv Station Richmond, VA 23298
CODEN
BLCTDO
Publication Date
19890101
Document Type
Journal Article
Funding Amount
913524.00
Funding Type
Grant
Fiscal Year
1989
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-02148
Issue of Publication
4
Priority Area
Reproductive-system-disorders
Source Name
Bioelectromagnetics
State
VA
Performing Organization
Virginia Commonwealth University, Richmond, Virginia
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