In vitro fertilization of mouse ova by spermatozoa exposed isothermally to radio-frequency radiation.
Cleary-SF; Liu-LM; Graham-R; East-J
Bioelectromagnetics 1989; 10(4):361-369
Mouse spermatozoa were exposed isothermally to radio frequency (RF) electromagnetic radiation and tested for ability to fertilize mouse ova in-vitro. Continuous wave RF radiation at 27 or 2450 megahertz (MHz) was administered for 1 hour to solutions of 10(3) to 10(4) motile sperm per milliliter, at 37 degrees-C. Sperm were prepared from CD1-breeder-mice and ova were obtained from F(1)(C57B1/6 X C3H)- mice. Gametes were coincubated at 37 degrees-C for 4.5 hours, ova were washed free of adherent sperm, fixed and examined by phase contrast microscopy for evidence of fertilization. Exposure to 27MHz radiation at the specific absorption rate (SAR) of 50 or 90 watts/kilogram (W/kg) caused significant reductions in fertilization of 50 and 27 percent. For 2450MHz RF radiation, reductions in fertilization of 38 and 52 percent were observed for 50 and 80W/kg, respectively. Electron microscopy revealed no apparent effects of RF radiation on sperm morphology, ultrastructure, or capacitation. The authors conclude that the significant reduction of in-vitro fertilization rates observed when spermatozoa are exposed to 27 or 2450MHz continuous wave radiation at SARs of 50W/kg or greater is not due to an increase in temperature, nor to a decrease in sperm motility. The applicability of the data to human RF exposure was discussed.
NIOSH-Publication; NIOSH-Grant; Electromagnetic-radiation; Radiation-exposure; In-vitro-studies; Fertility; Reproductive-effects; Laboratory-animals; Reproductive-system-disorders; Radio-waves; Radiation-hazards
Physiology and Biophysics Medical College of Virginia Box 694, Mcv Station Richmond, VA 23298
Virginia Commonwealth University, Richmond, Virginia