Chlordecone-induced follicular toxicity in mouse ovaries.
Reprod Toxicol 1989 Jan; 3(3):203-206
A detailed histologic assessment of the ovary was made following exposure of the adult virgin female CD-1-mouse to chlordecone (143500) for 4 weeks, 5 days a week at 0.25 milligrams/day in efforts to detect a morphological basis for the decreased ovulatory response observed following exogenous gonadotropin stimulation. A second group of mice were administered estradiol-17beta (50282) at oil only. At 24 hours after the last exposure the animals were sacrificed. Follicular development was classified as small, medium, or large following serial sectioning of the ovaries. In the estradiol-17beta treated group twice as many medium sized follicles were found (mean 231.9) as in both the chlordecone exposed (mean 190.1) and control (mean 116.2) groups. A much higher percent of atresia was noted in the large follicles in both the pesticide exposed and the estradiol-17beta exposed mice. There were more actual healthy, large follicles in the estradiol-17beta exposed group (mean 25.4) than the chlordecone exposed group (mean 18.5), however. The increased atresia among large follicles caused by both chlordecone and estradiol-17beta treatment may have been due to the estrogenicity of these agents. In chlordecone treated mice, a decreased pool of healthy large and medium sized follicles occurred. This was not observed in the estradiol-17beta treated mice. The authors suggest that the pool of potentially ovulatory follicles was reduced in the pesticide treated mice.
NIOSH-Publication; NIOSH-Grant; Laboratory-animals; Chlorinated-hydrocarbons; Pesticides; Agricultural-chemicals; Reproductive-system-disorders; Hormones; Reproductive-effects; In-vivo-studies
Anatomy Louisiana State University 1542 Tulane Avenue New Orleans, LA 70112
Louisiana State University, School of Medicine, Department of Anatomy, New Orleans, Louisiana