Isolation of metal-binding agents from coal dust and their effects on mitochondrial function.
Elia-VJ; Murthy-L; Petering-HG
Environ Lett 1973 Oct-Dec; 5(4):237-248
A study was undertaken to develop a method for extracting coal dust, to establish whether the extracts contained metal binding agents, and to gather evidence that these agents may interfere with trace metal metabolism or act as inhibitors of metalloenzymes requiring biological systems. Liver mitochondrial samples were prepared using Sprague-Dawley-rats. Two types of bituminous coal were used. The first, from Pennsylvania, was a harder variety, contained more heavy metals and caused a higher incidence of coal miner's pneumoconiosis than the second sample which was from Utah. The organic extract from the Utah sample contained material equivalent to 58 to 70 milligrams/gram (mg/g) of coal. The Pennsylvania sample extract contained material equivalent to 12 to 15mg/g of coal. The whole organic extract from each coal was chromatographed on carboxymethyl- cellulose in Cu(II) form (CMC-CuII). For Utah and Pennsylvania samples, the copper complexing fractions were 30 and 22 percent, respectively, of the total extract. Copper binding capacity was 400 micrograms copper/gram of Utah coal and 40 micrograms/gram Pennsylvania coal. The oxidative phosphorylation of isolated rat liver mitochondria was uncoupled by copper binding ligands from both coals. The Pennsylvania sample had a higher inhibitory activity per gram of extracted material. The authors suggest that the extraction and absorption of metal binding agents from coal may adversely alter vital cellular energetics.
NIOSH-Publication; NIOSH-Grant; Grants-other; Mining-industry; Coal-mining; Pulmonary-system-disorders; Lung-disorders; Cell-function; Lung-cells; Metabolic-study; Coal-dust; Laboratory-animals; In-vitro-studies
Environmental Health University of Cincinnati Eden & Bethesda Avenues Cincinnati, Ohio 45219
University of Cincinnati, Cincinnati, Ohio