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Improved methods of producing precipitating Aspergillus antigens.

Authors
Burrell-R; Thomas-CK
Source
Ann Allergy 1977 Mar; 38(3):202-205
Link
NIOSHTIC No.
00189166
Abstract
Improved methods for the production of Aspergillus-fumigatus antigens which are capable of giving sensitive and reproducible results by counterimmunoelectrophoresis (CIEP) were described. The influence of the type of casein hydrolysate used, the influence of the method of incubating the CIEP slide, the effect of freeze pressing and concentrating antigen preparations, the effect age of culture, and the effect of carbon and nitrogen source concentrations on antigen production were examined. Of the three different commercial sources used, polypeptone, neopeptone, and multi-peptone, only the antigen produced with polypeptone formed at least four lines without concentration when tested by CIEP with the reference antiserum. The other two antigens formed no discernible lines on CIEP. With regard to incubation time, several more fine lines were apparent when incubation was extended to 18 hours at 4 degrees-C. Concentrating the clarified extract five fold produced a few more lines but, more importantly, the procedure resulted in stronger lines of precipitation, particularly among the minor antigen antibody reaction lines. Older cultures resulted in preparations yielding fewer lines than younger cultures. The authors conclude that reliable Aspergillus antigens can easily be produced in the clinical laboratory.
Keywords
NIOSH-Publication; NIOSH-Grant; Pulmonary-system-disorders; Diagnostic-techniques; Fungi; Antigens
Contact
Microbiology West Virginia University Med C Department of Microbiology Morgantown, W VA 26506
CODEN
ANAEA3
Publication Date
19770301
Document Type
Journal Article
Funding Amount
289015
Funding Type
Grant
Fiscal Year
1977
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-000360
Issue of Publication
3
ISSN
0003-4738
Priority Area
Pulmonary-system-disorders
Source Name
Annals of Allergy
State
WV
Performing Organization
West Virginia University, Morgantown, West Virginia
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