Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Uroporphyrin I stimulation of collagen biosynthesis in human skin fibroblasts. A unique dark effect of porphyrin.

Authors
Varigos-G; Schiltz-JR; Bickers-DR
Source
J Clin Invest 1982 Jan; 69(1):129-135
NIOSHTIC No.
00187020
Abstract
A study was undertaken to assess the effects of uroporphyrin-I and of light on collagen synthesis in fibroblasts obtained from explant cultures of normal adult human skin. Fibroblast monolayers were preincubated for 24 hours in the dark with or without uroporphyrin-I and then incubated for 24 hours with tritium labeled proline. Accumulation of nondialyzable isotopically labeled hydroxyproline increased 2.7 fold when uroporphyrin-I was included in the incubation medium. This represented a specific increase in collagen synthesis. This increase was time dependent and occurred either in the presence or absence of ultraviolet light. A study was performed to determine whether the increased collagen accumulation in the presence of uroporphyrin-I might be the result of decreased collagen degradation. The study of the effects of uroporphyrin-I and light on the growth of human fibroblasts indicated the growth curves were similar for all cultures including the controls, light without porphyrin, porphyrin without light and porphyrin irradiated daily with 400 nanometer light. There were no obvious differences in cellular morphology in the cultures when examined by phase contrast microscopy. The authors suggest that the sclerodermatous lesions seen in the skin of patients with porphyria cutanea tarda and erythropoietic porphyria may be the result of the novel dark effect of uroporphyrin-I as demonstrated in this study.
Keywords
NIOSH-Publication; NIOSH-Grant; Dermatitis; Skin-exposure; Ultraviolet-radiation; In-vitro-studies; Metabolic-study; Liver-disorders
Contact
Medicine Cleveland V a Hospital 10701 East Blvd Cleveland, Ohio 44106
CODEN
JCINAO
Publication Date
19820101
Document Type
Journal Article
Funding Amount
267904
Funding Type
Grant
Fiscal Year
1982
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-01149
Issue of Publication
1
ISSN
0021-9738
Priority Area
Dermatitis
Source Name
Journal of Clinical Investigation
State
OH
Performing Organization
Case Western Reserve University, Cleveland, Ohio
TOP