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The dependence of male reproductive toxicity on germ cell stage.

Authors
Gandy-J; Teaf-CM; Adatsi-FA; James-RC; Harbison-RD
Source
Functional Teratogenesis: Functional Effects on the Offspring after Parental Drug Exposure. Fujii T, Adams PM, eds., Tokyo, Japan: Teikyo University Press, 1987 Jan; :133-145
Link
NIOSHTIC No.
00187014
Abstract
A series of experiments were conducted to investigate the dependence of male reproductive toxicity on germ cell stage in rats. Glutathione (GSH) levels were determined in segments of the reproductive tract in sexually mature male Fischer-344-rats with or without treatment with chemicals thought to have reproductive effects. Sexually mature male and female rats were used in a serial mating study of dominant lethal mutations. Clastogenic damage in male germ cell stage dependent dominant lethal mutations results from exposure to ethyl-methanesulfonate (62500) (EMS). The most damage was induced among spermatozoa in late spermatid and early spermatozoal development. Germ cell stage dependent activity of EMS was potentiated by both L-buthionine-sulfoximine (BSO) and 1,2- dibromoethane (106934) (EDB). Both BSO and EDB significantly reduced the epididymal glutathione content, likely the reason for the potentiation effect. At 8 and 16 hours post injection BSO treatment significantly depressed GSH in the epididymis and testes. EDB treatment significantly depressed GSH at 2 hours in caput and cauda epididymis but not in the testes. Trimethyl-phosphate (512561) (TMP) was also able to produce clastogenic damage as well as functionally impair spermatozoa in a dose dependent manner. This impairment was also dependent on germ cell stage. Mature spermatozoa were about four times more susceptible to the actions of TMP, resulting in altered motility. TMP treatment caused a 20 percent reduction in cauda epididymal spermatozoa carnitine- acetyltransferase activity. The authors conclude that overt chemical induced male reproductive toxicity may be dependent on germ cell stage but potency is markedly altered by biochemical influences.
Keywords
NIOSH-Grant; Reproductive-system-disorders; Laboratory-animals; Alkylating-agents; Cell-function; Toxic-effects; Reproductive-hazards
Contact
Pharmacology University of Arkansas 4301 W Markham Little Rock, Ark 72205
CAS No.
62-50-0; 106-93-4; 512-56-1
Publication Date
19870101
Document Type
Book or book chapter
Editors
Fujii-T; Adams-PM
Funding Amount
37671
Funding Type
Grant
Fiscal Year
1987
NTIS Accession No.
NTIS Price
ISBN No.
4990007628
Identifying No.
Grant-Number-R03-OH-02258
Priority Area
Reproductive-system-disorders
Source Name
Functional Teratogenesis: Functional Effects on the Offspring after Parental Drug Exposure
State
AR
Performing Organization
University of Arkansas Med Scis Ltl Rock, Little Rock, Arkansas
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