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Conversion of leukotriene A4 to leukotriene B4: Catalysis by human liver microsomes under anaerobic conditions.

Authors
Gut-J; Goldman-DW; Jamieson-GC; Trudell-JR
Source
Arch Biochem Biophys 1987 Dec; 259(2):497-509
NIOSHTIC No.
00187002
Abstract
The presence of a microsomal enzyme that catalyzes leukotriene-A4 (LTA4) hydrolysis was demonstrated in human liver. The first application of the new technique of direct chemical ionization mass spectroscopy to the identification and characterization of underivatized leukotriene-B4 (LTB4) was also described. A 2 minute incubation of LTA4 with human liver microsomes allowed for the conversion of 1.7 mole percent to LTB4. Protein concentration, time, and substrate concentration were determining factors in this conversion. The conversion was not supported by heat inactivated microsomes nor did it require NADPH. Apparent Michaelis-Menten type behavior was determined through the kinetic analysis of the reaction. Among three patients examined, the production rates varied widely. LTB4 formation by microsomal LTA4 hydrolase was inhibited 52, 40, and 60 percent by piperonyl-butoxide, propanethiol, and cyclohexene-oxide, respectively. In the presence of 100 percent oxygen the activity of microsomal and cytosolic LTA4- hydrolase was decreased by 45 and 64 percent, respectively. The mass spectrum of 50 nanograms of underivatized synthetic LTB4 free acid was identical with that of 10 nanograms of the product isolated from LTA4 hydrolysis by human liver microsomes.
Keywords
NIOSH-Publication; NIOSH-Grant; Grants-other; Enzyme-activity; Microsomal-enzymes; Liver-microsomes; Fatty-acids; In-vitro-studies
Contact
Anesthesia Stanford University Department of Anesthesia Stanford, Calif 94305
CODEN
ABBIA4
Publication Date
19871201
Document Type
Journal Article
Funding Amount
1801197
Funding Type
Grant
Fiscal Year
1988
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-00978
Issue of Publication
2
ISSN
0003-9861
Priority Area
Other Occupational Concerns; Grants-other
Source Name
Archives of Biochemistry and Biophysics
State
CA
Performing Organization
Stanford University, Stanford, California
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