Cell-mediated immunity to soluble and particulate inhaled antigens.
Clin Exp Immunol 1979 Nov; 38(2):332-341
Cell mediated immunity to soluble and particulate inhaled antigens was studied in guinea-pigs. English-White-guinea-pigs were exposed by inhalation to aerosols of soluble or particulate human-serum- albumin (9048468) (HSA) 1 hour every fourth day for up to 4 weeks. Soluble HSA was a 200 micrograms per milliliter solution of HSA in phosphate buffered saline, pH 7.2. Particulate HSA was HSA conjugated to respirable carboxylated latex beads. Controls were unexposed or animals inhaling unconjugated latex beads only. Selected animals were killed at weekly intervals and the lungs were removed and lavaged. Lavage fluid total and differential cellularity was determined. Lymphoid cells were recovered from the lavage fluid and tested for their ability to proliferate in response to phytohemagglutinin (PHA) and concanavalin-A (conA). Peripheral blood lymphocytes were collected and also tested for their ability to respond to PHA and conA. The indirect macrophage migration inhibition (MMI) and the transformation by antigen stimulation (TBAS) tests were also performed. The largest number of free lung cells was obtained from guinea-pigs exposed to particulate HSA, followed by those exposed to unconjugated latex beads, soluble HSA, and those unexposed in that order. The cells consisted primarily of macrophages and lymphocytes in each case. Only pulmonary lymphocytes from guinea-pigs exposed to soluble HSA showed a significant response to PHA or conA. Peripheral blood lymphocytes from all groups reacted to PHA and conA. No consistent differences between the groups were seen because of great inter animal variability. Only guinea-pigs exposed to particulate HSA consistently responded in the TBAS and MMI assay. Some soluble HSA exposed guinea-pigs showed a positive response in the MMI test after 2 weeks. When lavage cells with depleted macrophage populations were used, the response in the MMI test was decreased. The authors suggest that alveolar macrophages at in-vivo concentrations can modulate the reactivity of pulmonary lymphocytes to mitogens and antigens. The antigenicity of inhaled antigens may depend on the type and nature of cells responding to the stimulus.
NIOSH-Publication; NIOSH-Grant; Pulmonary-system-disorders; In-vivo-studies; Particulates; Laboratory-animals; In-vitro-studies; Lung-cells; Blood-cells; Immunology; Inhalation-studies; Physiological-response
Microbiology West Virginia University Med C Department of Microbiology Morgantown, W VA 26506
Clinical and Experimental Immunology
West Virginia University, Morgantown, West Virginia