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Effects of purified altertoxins I, II, and III in the metabolic communication V79 system.

Authors
Boutin-BK; Peeler-JT; Twedt-RM
Source
J Toxicol Environ Health 1989 Jan; 26(1):75-81
NIOSHTIC No.
00186606
Abstract
The effects of altertoxins on metabolic communication were studied in-vitro. Purified altertoxin-I (56258323), altertoxin-II (56257591), or altertoxin-III (105579746) prepared from Alternaria- alternata grown on cooked rice were incubated with Chinese-hamster- V79 cells at concentrations of 0 to 5 micrograms per milliliter (microg/ml). The compounds were evaluated for their ability to inhibit gap junction communication in a 6-thioguanine resistance assay. Cytotoxicity was evaluated by determining doses that allowed 70 to 95 percent colony survival. 12-O-tetradecanoylphorbol-13- acetate (TPA) and 4-O-methyl-ether-tetradecanoylphorbol-13-acetate (4OTPA) were used as positive controls. Altertoxin-II was the most cytotoxic, followed by altertoxin-III and altertoxin-I. Altertoxin- I was significantly more disruptive to metabolic communication that 4OTPA at all concentrations; the greatest effect was shown by the 4 and 5microg/ml doses. Altertoxin-I weakly affected metabolic communication compared to TPA. Altertoxin-II and altertoxin-III did not significantly affect gap junction communication. The authors conclude that altertoxins induce dose dependent cytotoxicity and altertoxin-I weakly inhibits metabolic communication. Altertoxins that cause significant cytotoxicity or which interfere with cell to cell communication may adversely affect populations which are exposed to altertoxin contaminated food supplies.
Keywords
NIOSH-Author; In-vitro-studies; Mammalian-cells; Cellular-function; Natural-products; Fungi; Biochemical-indicators; Dose-response
CODEN
JTEHD6
CAS No.
56258-32-3; 56257-59-1; 105579-74-6
Publication Date
19890101
Document Type
Journal Article
Fiscal Year
1989
NTIS Accession No.
NTIS Price
Issue of Publication
1
ISSN
0098-4108
Source Name
Journal of Toxicology and Environmental Health
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