Acid and alkaline phosphatase activity in migrating primordial germ cells of the early chick embryo.
Anat Rec 1982 Mar; 202(3):379-385
The disposition of alkaline and acid-phosphatase activity was examined in the primordial germ cells (PGCs) of the chick embryo during their migration from extraembryonic areas to the gonads in order to assess the metabolic activity of these cells during this critical early developmental period. White-Leghorn-chick embryos were sacrificed at days one through six of incubation. No specific acid-phosphatase activity was noted in 1 and 2 day old embryos. PGCs were noted in the vascular system by the third incubation day, however, most had left the intraembryonic circulatory system and entered the dorsal mesentery; the circulating PGCs were negative for acid-phosphatase activity. On days four and five many PGCs were in the dorsal mesentery preparing to enter the gonads; a few PGCs demonstrated a positive reaction for acid-phosphatase, but most of these were located some distance from the gonad. The fifth and sixth day studies indicated that most PGCs were within the developing gonads and showed no acid-phosphatase activity. Alkaline- phosphatase activity was noted at 2 days of incubation in PGCs during the passive phase of their migration in extraembryonic blood vessels. Alkaline-phosphatase positive PGCs were also observed in the dorsal mesentery during the active phase of migration; however, the cellular localization of this enzyme differed from that observed in the passively migrating PGCs. According to the authors, the differences in distribution of alkaline-phosphatase indicate that there is a change occurring in these cells during these two migration stages.
NIOSH-Publication; NIOSH-Grant; Reproductive-system-disorders; Cell-function; Enzyme-activity; Metabolic-study; Embryology
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The Anatomical Record
Louisiana State University, School of Medicine, Department of Anatomy, New Orleans, Louisiana