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Metabolism of nitrous oxide.

Authors
Trudell-JR
Source
Nitrous Oxide/N2O. Eger EI II., ed., New York: Elsevier, 1985 Jan; :203-210
Link
NIOSHTIC No.
00185745
Abstract
Studies on the metabolism of nitrous-oxide (10102439) were conducted using nitrous-oxide labeled with the stable isotope nitrogen-15. Isotope ration mass spectrometry was used to quantify metabolically produced nitrogen-15. Animal studies indicated that nitrous-oxide was not apparently metabolized by either liver microsomes or liver homogenates. Even so, nitrous-oxide was metabolized through a reductive pathway by anaerobic bacteria from the human intestine. Nitrous-oxide also appeared to be an intermediate in the reduction of nitrite to nitrogen by bacteria. Incubations of nitrite and nitric-oxide molecules with bacteria produced nitrogen gas that contained a nitrogen atom from both nitrite and nitric-oxide molecules. For the reduction of nitrous-oxide to nitrogen, it may be necessary for a copper containing enzyme to be present. Otherwise, nitrous-oxide would accumulate. During the metabolic process free radicals were formed which could produce toxic agents such as peroxidized lipids. The toxicity of the free radicals was suggested by studies of an aqueous solution of nitrous-oxide that was irradiated by x-rays. The irradiated solution was lethal to Escherichia-coli. Experiments were designed to detect the free radicals produced during nitrous-oxide metabolism. While it appeared that nitrous-oxide radical formation could produce toxic effects, the known toxicity of nitrous-oxide could be ascribed to its inactivation of vitamin-B12.
Keywords
NIOSH-Grant; Grants-other; Gastrointestinal-system; Metabolic-study; Nitrogen-compounds; Enzyme-activity
Contact
Anesthesia Stanford University Department of Anesthesia Stanford, Calif 94305
CAS No.
10102-43-9
Publication Date
19850101
Document Type
Book or book chapter
Editors
Eger-EI II.
Funding Amount
1801197
Funding Type
Grant
Fiscal Year
1985
NTIS Accession No.
NTIS Price
ISBN No.
9780444008602
Identifying No.
Grant-Number-R01-OH-00978
Priority Area
Other Occupational Concerns; Grants-other
Source Name
Nitrous Oxide/N2O
State
NY; CA
Performing Organization
Stanford University, Stanford, California
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