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Ultrastructural studies of the dying-back process. V. axonal neurofilaments accumulate at sites of 2,5-hexanedione application: evidence for nerve fibre dysfunction in experimental hexacarbon neuropathy.

Authors
Politis-MJ; Pellegrino-RG; Spencer-PS
Source
J Neurocytol 1980 Aug; 9(4):505-516
NIOSHTIC No.
00185573
Abstract
The production of distal axonopathy by the direct toxic effects of 2,5-hexanedione (110134) (25HD) on nerve fibers was assessed in Sprague-Dawley-rats. Sciatic nerves of the test animals were exposed and treated with 25HD, 2,4-hexanedione (3002242) (24HD), 1,6- hexanediol (629118) (16HDiol), saline, or an aqueous solution of hydrochloric-acid (7647010) (HCl) daily for periods of up to 8 days with and without oral 25HD treatment for 8 days prior to surgery. Nerves were examined by electron microscopy at times between 6 hours and 16 days post treatment. Oral exposure to 25HD in the drinking water and the direct application of 25HD to the sciatic nerve produced unilateral hindlimb weakness concomitant with nerve fiber breakdown at the site of application and diffuse Wallerian degeneration of the distal zone. Light microscopy revealed swollen myelinated and demyelinated axons with a similar appearance to the giant axonal swellings characteristic of systemic hexacarbon neuropathy. Electron microscopic examination at 4 and 16 days post treatment showed swollen demyelinated axons filled with 10 nanometer neurofilaments and containing scattered clusters of microtubules and Schwann cell necrosis. No qualitative differences were determined for oral versus direct nerve exposure to the agent, but animals exposed to 25HD in the drinking water and by direct nerve application had a higher incidence of axonal swellings. Treatment of the nerve fibers with 24HD produced a nonspecific breakdown of nerve tissue and Schwann cells without any significant effect on the axonal distribution of neurofilaments and microtubules. Treatment with 16HDiol was without effect. The authors conclude that 25HD can induce the formation of giant axonal swellings through direct toxic action and that the Schwann cell changes observed after application of 25HD or 24HD are nonspecific and not related to axonal swelling.
Keywords
NIOSH-Publication; NIOSH-Grant; Neurotoxic-effects; Microscopic-analysis; Histopathology; Organic-solvents; Nerve-damage; Peripheral-nervous-system
Contact
Pathology Albert Einstein Coll of Med 1300 Morris Park Avenue Bronx, N Y 10461
CODEN
JNCYA2
CAS No.
110-13-4; 3002-24-2; 629-11-8; 7647-01-0
Publication Date
19800801
Document Type
Journal Article
Funding Amount
807725
Funding Type
Grant
Fiscal Year
1980
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-00535
Issue of Publication
4
ISSN
0300-4864
Priority Area
Neurotoxic Disorders; Neurotoxic-effects
Source Name
Journal of Neurocytology
State
NY
Performing Organization
Yeshiva University, New York, New York
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