Role of gap junctions in adverse reproductive outcome.
University of Michigan, Ann Arbor, Michigan 1988 Nov; :1-26
Several toxicants were evaluated for their effects on junctional communication between cells. Various techniques were used to measure junctional communication, including a new, rapid and sensitive dye coupling assay. The V79 cell line was used in most of the experiments, derived from Chinese-hamster embryo lung, along with the HT cell line, derived from a human teratocarcinoma and the SK-UT-1 cell line, derived from a human leiomyosarcoma of the uterus. Responses to reproductive and developmental toxicants including dieldrin (60571), ethylene-glycol-monomethyl-ether, ethylene-glycol-monoethyl-ether, ethanol (64175), lead (7439921), mercury (7439976), nickel (7440020), zinc (7440666), and arsenic (7440382) were monitored. Junctional communication was inhibited in at least one of the cell lines by every substance tested except nickel and zinc. Work has begun to determine the effects of calcium (7440702) and cAMP on junctional communication in the SK-UT-1 cells. The findings suggest that cAMP may modulate gap junctions by multiple mechanisms, including the synthesis of new protein. Studies also used the hydra, a simple invertebrate, to evaluate junctional communication in a whole animal, morphogenetic system.
NIOSH-Grant; Reproductive-system-disorders; Organo-chlorine-compounds; Pesticides; Agricultural-chemicals; Solvents; Metallic-poisoning
Environmental & Indust Health University of Michigan 109 Observatory Street Ann Arbor, MI 48109-2029
60-57-1; 64-17-5; 7439-92-1; 7439-97-6; 7440-02-0; 7440-66-6; 7440-38-2; 7440-70-2
Final Grant Report
NTIS Accession No.
University of Michigan, Ann Arbor, Michigan
University of Michigan at Ann Arbor, Ann Arbor, Michigan