Toxicity of 1,2-dibromoethane in primary hepatocyte monolayer cultures: Lack of dependence on oxygen concentration.
Toxicol Appl Pharmacol 1988 Sep; 95(2):241-247
The role of oxygen concentration in the delayed toxicity of 1,2- dibromoethane (106934) (DBE) in primary hepatocyte cultures was investigated. Hepatocytes were isolated from male Fischer-344-rats and grown in monolayer for 18 hours before use. DBE was delivered to cells as a vapor over a 2 hour period in concentrations of 0, 14, 140, 1400, and 14000 parts per million (ppm). Atmospheres containing 1, 2, or 20 percent oxygen were tested. Cytotoxicity, assessed as trypan-blue exclusion and aspartate-aminotransferase leakage, was measured immediately after exposure and 24 hours later. Dose related DBE toxicity was noted at 24 hours after exposure and was unaffected by oxygen concentration. The median lethal concentration immediately after exposure was 14000ppm and was 140ppm by 24 hours post exposure, indicating that toxicity was initiated by DBE that was not apparent immediately after exposure. There was a threshold exposure level between 14 and 140ppm DBE for the 2 hour exposure, from which cells could no longer recover. The authors conclude that hypoxic conditions do not appear to play a role in the acute toxicity of DBE; thus, lipid peroxidation may not be an important factor in DBE toxicity.
NIOSH-Publication; NIOSH-Grant; Grants-other; In-vitro-studies; Liver-cells; Laboratory-animals; Brominated-ethanes; Toxic-effects; Hypoxia
Anesthesia Stanford University Department of Anesthesia Stanford, Calif 94305
Other Occupational Concerns; Grants-other
Toxicology and Applied Pharmacology
Stanford University, Stanford, California