Techniques for the quantitative analysis of the particulate burden in lung tissue were reviewed. Methods for tissue processing were based on determination of whether the particles should be analyzed in-situ or after isolation from the tissue. The in-situ analysis of particles utilized light microscopy, scanning electron microscopy, electron microprobe x-ray analysis, and ion probe microanalysis. Isolation methods were used when the tissue components interfered with particle analysis or when the particles were present at very low concentrations. Specific isolation techniques were dependent upon the concentration and nature of the particles to be analyzed. Physical and chemical methods of tissue destruction were described. Particle separation from the decomposed tissue was achieved by filtration and/or centrifugation with or without prior solubilization of the particulate matter. Particle by particle analysis and bulk analytical methods were available for quantitative analysis. Bulk analytical techniques included wet chemical methods, atomic emission, atomic absorption, infrared spectroscopy, and x-ray diffraction. Particle by particle analysis employed electron microscopic techniques. Particle identification, particle counting, and elemental versus molecular analysis were discussed.