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Lung collagen in silicosis: fibrosis mechanisms.

Authors
Reiser-KM
Source
Department of Internal Medicine, University of California, Davis, California, 1987 Aug; :1-4
Link
NIOSHTIC No.
00182724
Abstract
The mechanisms involved in lung fibrosis in silicosis were investigated in rats. Studies were carried out to test whether collagen crosslinking in silicotic rat lungs differs from that found in normal lungs; to determine whether crosslinking in silicotic rat lungs differs from that in bleomycin exposed rat lungs; to determine whether elastin crosslinking can be measured in the lungs of either normal adult rats or rats exposed to fibrogenic stimuli and if there are differences among the groups; and to develop simple screening assays of urine or blood to indicate early silicotic changes. Silicotic collagen was characterized by an increase in the ratio of the difunctional crosslinks DHLNL to HLNL, an increase in the content of the trifunctional nonreducible crosslink hydroxypyridinium and an apparent increase in lysine hydroxylation. Long term bleomycin experiments indicated further evidence that increased hydroxypyridinium may serve as a permanent marker of a fibrotic event that may have occurred months to years earlier. The sensitive high pressure liquid chromatographic method developed for the detection of desmosine indicated no differences in desmosine in silicotic animals as compared to controls. No consistent differences in crosslink excretion were noted between control and silicotic animals. The authors conclude that any small changes in crosslink excretion contributed by damaged lungs were obscured by the larger contributions of the urine crosslink pool of such tissues as bone.
Keywords
NIOSH-Grant; Pulmonary-system-disorders; Lung-disorders; Laboratory-animals; Blood-analysis; Urine-chemistry
Contact
Internal Medicine California Primate Res Center University of California Davis, CA 95616
Publication Date
19870801
Document Type
Final Grant Report
Funding Amount
97200
Funding Type
Grant
Fiscal Year
1987
NTIS Accession No.
PB89-130744
NTIS Price
A01
Identifying No.
Grant-Number-K01-OH-00002
NIOSH Division
OEP
Priority Area
Pulmonary-system-disorders
Source Name
Department of Internal Medicine, University of California, Davis, California
State
CA
Performing Organization
University of California Davis, Davis, California
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