Lung collagen in silicosis: fibrosis mechanisms.
Department of Internal Medicine, University of California, Davis, California, 1987 Aug; :1-4
The mechanisms involved in lung fibrosis in silicosis were investigated in rats. Studies were carried out to test whether collagen crosslinking in silicotic rat lungs differs from that found in normal lungs; to determine whether crosslinking in silicotic rat lungs differs from that in bleomycin exposed rat lungs; to determine whether elastin crosslinking can be measured in the lungs of either normal adult rats or rats exposed to fibrogenic stimuli and if there are differences among the groups; and to develop simple screening assays of urine or blood to indicate early silicotic changes. Silicotic collagen was characterized by an increase in the ratio of the difunctional crosslinks DHLNL to HLNL, an increase in the content of the trifunctional nonreducible crosslink hydroxypyridinium and an apparent increase in lysine hydroxylation. Long term bleomycin experiments indicated further evidence that increased hydroxypyridinium may serve as a permanent marker of a fibrotic event that may have occurred months to years earlier. The sensitive high pressure liquid chromatographic method developed for the detection of desmosine indicated no differences in desmosine in silicotic animals as compared to controls. No consistent differences in crosslink excretion were noted between control and silicotic animals. The authors conclude that any small changes in crosslink excretion contributed by damaged lungs were obscured by the larger contributions of the urine crosslink pool of such tissues as bone.
NIOSH-Grant; Pulmonary-system-disorders; Lung-disorders; Laboratory-animals; Blood-analysis; Urine-chemistry
Internal Medicine California Primate Res Center University of California Davis, CA 95616
Final Grant Report
NTIS Accession No.
Department of Internal Medicine, University of California, Davis, California
University of California Davis, Davis, California