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Detection of styrene oxide-DNA adducts by 32P-postlabeling.

Authors
Liu-F; Rappaport-SM; Rasmussen-J; Bodell-WJ
Source
Carcinogenesis 1988 Aug; 9(8):1401-1404
NIOSHTIC No.
00182076
Abstract
The formation of DNA adducts by styrene-7,8-oxide (96093) (styrene- oxide) was studied in-vitro. One to 30 microliters (microl) of styrene-oxide were reacted with 0.5 milligram calf thymus DNA, 2'- deoxyguanosine-3'-monophosphate (dGp), 2'-deoxyadenosine-3'- monophosphate (dAp), 2'-deoxycytidine-3'-monophosphate (dCp), or 2'- deoxythymidine-3'-monophosphate (dTp) in a Tris/hydrochloric-acid buffer, pH 7.5, at 37 degrees-C for about 15 hours. The mixtures were assayed for adducts using a phosphorus-32 (P-32) postlabeling technique. Five styrene-oxide/DNA adducts were detected. The overall extent of DNA modification by 5microl styrene-oxide was approximately 4.4x10(-4) adducts per nucleotide. Styrene-oxide formed five adducts with dGp and two with dAp. Thin layer chromatography of the adducts on polyethyleneimine plates showed that the styrene-oxide/DNA adducts were similar to the corresponding DNA adducts. No adducts were detected with dCp or dTp. Additionally 9L cells were treated with 1 millimolar styrene-oxide for 24 hours. The cells were examined for DNA adducts as before. Several adducts were detected; the overall extent of DNA modification by styrene-oxide amounted to 19.5x10(-6) adducts/nucleotide. The adducts seem to be similar to those formed with calf thymus DNA. The authors conclude that guanine is the major site for DNA modification by styrene-oxide. The P-32 postlabeling technique can be used to detect formation of styrene- oxide/DNA adducts.
Keywords
NIOSH-Publication; NIOSH-Grant; Grants-other; Nucleic-acids; Chemical-binding; In-vitro-studies; Molecular-structure; Epoxides; Organic-chemicals; DNA-adducts
Contact
Biomedical & Environ Hlth Scis University of California School of Public Health Berkeley, CA 94720
CODEN
CRNGDP
CAS No.
96-09-3
Publication Date
19880801
Document Type
Journal Article
Funding Amount
838959
Funding Type
Grant
Fiscal Year
1988
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-02221
Issue of Publication
8
ISSN
0143-3334
Priority Area
Other Occupational Concerns; Grants-other
Source Name
Carcinogenesis
State
CA
Performing Organization
University of California Berkeley, Berkeley, California
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