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Pathogenesis of occupationally-induced isocyanate reactions.

Authors
Bernstein-IL
Source
University of Cincinnati Medical Center, Cincinnati, Ohio, Terminal Progress Report, 1982 Aug; :1-4
Link
NIOSHTIC No.
00181317
Abstract
An animal model of isocyanate induced hypersensitivity was sought. Several isocyanate/protein conjugates were prepared using strain B6D2F1-mice concerning the induction of homocytotropic antibody suggested that the murine model of isocyanate induced homocytotropic antibody would be a convenient method of assessing the reliability and reproducibility of various isocyanate/protein reagents. Further studies using guinea-pigs indicated that antigenic determinants in toluene-diisocyanate (26471625) (TDI) or hexamethylene-diisocyanate (822060) (HDI) conjugates were hapten specific. Carrier protein specificity was also demonstrated. No evidence of a specific cross reactivity between TDI and HDI was found. Gel diffusion studies indicated that TDI/HSA antisera cross reacted with HDI/HSA antisera, but not to HSA alone, suggesting new antigenic determinants in the isocyanate conjugates may determine cross reactivity between TDI/HSA and HDI/HSA conjugates. Experiments dealing with cell mediated immunity to isocyanates were in progress. Early results suggest that specific cell mediated immunity can be induced by parenteral immunization of Hartley-guinea-pigs with isocyanate/protein conjugates emulsified in complete Freund's adjuvant. The author concludes that the guinea-pig is the best available animal model of isocyanate induced allergic reactions and that TDI allergic sensitization should be demonstrable under work simulated conditions.
Keywords
NIOSH-Grant; Respiratory-irritants; Laboratory-animals; Allergic-reactions; Immune-reaction; Skin-irritants
Contact
Internal Medicine University of Cincinnati 231 Bethesda Avenue Cincinnati, Ohio 45267
CAS No.
26471-62-5; 822-06-0
Publication Date
19820801
Document Type
Final Grant Report
Funding Amount
301375
Funding Type
Grant
Fiscal Year
1982
NTIS Accession No.
PB88-247283
NTIS Price
A01
Identifying No.
Grant-Number-R01-OH-00773
NIOSH Division
OEP
Source Name
University of Cincinnati Medical Center, Cincinnati, Ohio, Terminal Progress Report
State
OH
Performing Organization
University of Cincinnati, Cincinnati, Ohio
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