Pathogenesis of occupationally-induced isocyanate reactions.
University of Cincinnati Medical Center, Cincinnati, Ohio, Terminal Progress Report, 1982 Aug; :1-4
An animal model of isocyanate induced hypersensitivity was sought. Several isocyanate/protein conjugates were prepared using strain B6D2F1-mice concerning the induction of homocytotropic antibody suggested that the murine model of isocyanate induced homocytotropic antibody would be a convenient method of assessing the reliability and reproducibility of various isocyanate/protein reagents. Further studies using guinea-pigs indicated that antigenic determinants in toluene-diisocyanate (26471625) (TDI) or hexamethylene-diisocyanate (822060) (HDI) conjugates were hapten specific. Carrier protein specificity was also demonstrated. No evidence of a specific cross reactivity between TDI and HDI was found. Gel diffusion studies indicated that TDI/HSA antisera cross reacted with HDI/HSA antisera, but not to HSA alone, suggesting new antigenic determinants in the isocyanate conjugates may determine cross reactivity between TDI/HSA and HDI/HSA conjugates. Experiments dealing with cell mediated immunity to isocyanates were in progress. Early results suggest that specific cell mediated immunity can be induced by parenteral immunization of Hartley-guinea-pigs with isocyanate/protein conjugates emulsified in complete Freund's adjuvant. The author concludes that the guinea-pig is the best available animal model of isocyanate induced allergic reactions and that TDI allergic sensitization should be demonstrable under work simulated conditions.
NIOSH-Grant; Respiratory-irritants; Laboratory-animals; Allergic-reactions; Immune-reaction; Skin-irritants
Internal Medicine University of Cincinnati 231 Bethesda Avenue Cincinnati, Ohio 45267
Final Grant Report
NTIS Accession No.
University of Cincinnati Medical Center, Cincinnati, Ohio, Terminal Progress Report
University of Cincinnati, Cincinnati, Ohio