Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Anesthetic Metabolism - Toxic Effects in OR Personnel.

Authors
Trudell-JR
Source
Stanford University, Terminal Progress Report :6 pages
Link
NIOSHTIC No.
00181243
Abstract
Studies were conducted on the binding of halothane (151677) metabolites to human liver and gonads. Possible metabolism of nitrous-oxide (10024972) was also studied using isotope ratio mass spectrometry and a method of electron paramagnetic resonance spin trapping. A gas chromatograph/mass spectrometry technique was developed to identify and quantify the volatile metabolites of halothane produced in patients and in-vitro incubations. The most toxic metabolite was formed in the patient's breathing circuit, mainly from contact of halothane with hot, wet soda lime. Toxicity and mutagenicity studies, making use of a new log growth mutagenic assay particularly appropriate for assessing potentially mutagenic halocarbons, indicated that some of the reductive metabolites were moderately mutagenic. Efforts were undertaken to develop a synthetic system in which drug metabolism can be studied using highly purified single forms of cytochrome-P-450 proteins in a membrane environment of exactly defined composition and physical properties. An aim in the development of this reconstitution technique was the ability to use human liver cytochromes in this system and ultimately develop a system in which drug toxicity could be tested in human liver cell components without exposing humans to harmful drugs.
Keywords
NIOSH-Grant; Pulmonary-system-disorders; Pharmacodynamics; Metabolic-study; Anesthetics; Anesthesiology; Chromatographic-analysis; Mutagens; Liver-damage;
Contact
Anesthesia Stanford University 300 Pasteur Drive Stanford, Calif 94305
CAS No.
151-67-7; 10024-97-2;
Funding Amount
599304.00
Funding Type
Grant;
NTIS Accession No.
PB88-247945
NTIS Price
A02
Identifying No.
Grant-Number-R01-OH-00622
Priority Area
Pulmonary-system-disorders
Source Name
Stanford University, Terminal Progress Report
State
CA;
Performing Organization
Stanford University, Stanford, California
TOP