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Determination of urinary 2-ethoxyacetic acid as an indicator of occupational exposure to 2-ethoxyethanol.

Authors
Smallwood-AW; DeBord-K; Burg-J; Moseley-C; Lowry-L
Source
Appl Ind Hyg 1988 Feb; 3(2):47-50
NIOSHTIC No.
00177904
Abstract
A method for determining 2-ethoxyacetic-acid (627032) (EAA) in urine was described. The method was intended to detect exposure to 2- ethoxyethanol (110805) (EE). Urine samples were shaken with potassium-carbonate, tetrabutylammonium-hydrogen-sulfate, methylene- chloride, and pentafluorobenzyl-bromide for 2 hours, heated in a water bath at 50 degrees-C for 20 minutes, and shaken again for 1 hour. The aqueous layer was discarded. The methylene-chloride layer was washed twice with distilled water and then dried over anhydrous sodium-sulfate. A 4 microliter aliquot of the dried methylene- chloride extract was analyzed on a gas chromatograph with a flame ionization detector. A calibration curve prepared by spiking urine samples with EAA was linear over the range 5 to 100 micrograms per milliliter (microg/ml). The detection limit was 4microg/ml and the limit of quantitation was 7microg/ml. Day to day precision was 3.0 to 4.7 percent relative standard deviation (RSD) and intraday precision was less than 2 percent RSD. Urine samples were obtained from 27 painters using paints containing EE, five painters not using EE, and 20 unexposed shipyard workers and analyzed for EAA. Mean urinary EAA concentrations expressed as milligrams per gram creatinine were: painters not using EE, 6.6; painters using EE, 25.0; and shipyard workers, not detected. The authors conclude that the method is potentially useful for detecting occupational exposure to EE.
Keywords
NIOSH-Author; Analytical-methods; Chromatographic-analysis; Metabolites; Glycols; Ethers; Urinalysis; Biological-monitoring; Occupational-exposure; Chemical-extraction
CODEN
AIHYEX
CAS No.
627-03-2; 110-80-5
Publication Date
19880201
Document Type
Journal Article
Fiscal Year
1988
NTIS Accession No.
NTIS Price
Issue of Publication
2
ISSN
0882-8032
NIOSH Division
DBBS
Source Name
Applied Industrial Hygiene
State
OH; GA
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