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Lipid peroxidation in guinea pig lung microsomes.

Authors
Wright-JR; Colby-HD; Miles-PR
Source
Biochim Biophys Acta 1980; 619:374-384
NIOSHTIC No.
00176510
Abstract
Pulmonary microsomal lipid peroxidation was studied in-vitro with regard to the effects of NADPH, ferrous ion (Fe(2+)), ferric ion (Fe(3+)), and ascorbate. Lungs were dissected from male English- short-hair-guinea-pigs, and the microsomes were obtained from lung homogenates by differential centrifugation. Lipid peroxidation was determined by the production of malonaldehyde in the presence or absence of the substances used to initiate or modify peroxidation. The addition of 2.5 millimolar (mM) Fe(2+), Fe(3+), or ascorbate increased pulmonary microsomal lipid peroxidation in the increasing order of Fe(3+), ascorbate, and Fe(2+). The addition of ascorbate enhanced Fe(3+) stimulated lipid peroxidation but reduced the magnitude of the Fe(2+) stimulated lipid peroxidation. NADPH stimulated lipid peroxidation in normal pulmonary microsomes but not in heat treated preparations or preparations containing 1mM ethylenediaminetetraacetic-acid. Fe(2+) and phosphate were required for maximal microsomal stimulation by NADPH, but the addition of NADPH to the Fe(2+) medium decreased microsomal stimulation when the iron concentration was greater than 1mM. The results were compared to similar experiments using liver microsomal preparations, and the authors conclude that there are significant differences in the behavior of pulmonary and hepatic microsomes.
Keywords
NIOSH-Author; Enzymatic-effects; Microsomal-enzymes; Pulmonary-system; Lipid-peroxidation; Physiological-response; Laboratory-animals; Pulmonary-function; Lung-cells; In-vitro-studies
CODEN
BBACAQ
Publication Date
19800101
Document Type
Journal Article
Fiscal Year
1980
NTIS Accession No.
NTIS Price
Priority Area
Pulmonary-system
Source Name
Biochimica et Biophysica Acta
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