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Development of an in situ microbial mutagenicity test system for airborne workplace mutagens: laboratory evaluation.

Authors
Whong-Z; Stewart-J; Ong-T
Source
Mutat Res 1984 Feb; 130(1):45-51
NIOSHTIC No.
00176507
Abstract
An in-situ microbial mutagenicity test system for workplace airborne mutagen monitoring was described. The system involved trapping the mutagens by impingement followed by mutagenicity testing using the Salmonella histidine reversion and arabinose resistant tests. The trapping device consisted of a pump, an impinger, and a cyclone; the system drew test air into medium containing test bacteria. The tester strains used were the Salmonella-typhimurium testers that were resistant to streptomycin and 8-azaguanine, (TA-98W) and (TA- 100W), and the arabinose resistant tester, (SV-50W). These strains had comparable mutagenic sensitivities to 2-aminoanthracene and ICR- 191 as their parent strains. The microbial system with the new tester strains was tested using vapors of methyl-methanesulfonate (66273), ethyl-methanesulfonate (62500), and dimethylnitrosamine (62759). Mutagenicity increased as impingement time increased for all compounds and all three strains. The impingement method yielded a 62 to 77 percent recovery of methyl-methanesulfonate, and particle trapping properties were adequate as determined by tests with suspended silica particles coated with 2,4,7-trinitro-9-fluorenone (129793). Promutagen detection was accomplished by the incorporation of metabolic activation into the trapping medium through inclusion of S9 mix and tester cells in dialysis tubing. The authors conclude that this system could provide a simple and useful mutagenic monitor for workplace applications.
Keywords
NIOSH-Author; Industrial-environment; Monitoring-systems; Air-contamination; Microbial-test-systems; Mutagenesis; Industrial-hygiene; Occupational-hazards; Gene-mutation; Bioassays; Air-sampling
CODEN
MUREAV
CAS No.
66-27-3; 62-50-0; 62-75-9; 129-79-3
Publication Date
19840201
Document Type
Journal Article
Fiscal Year
1984
NTIS Accession No.
NTIS Price
Issue of Publication
1
ISSN
0027-5107
NIOSH Division
DRDS
Source Name
Mutation Research
State
WV
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