Isolation and Identification of Phagocytic Cells.
Castranova-V; Scott-MR; Dyke-K
Cellular Chemiluminescence 1987;:25-38
Methods for purifying phagocytic cells from various tissues were reviewed. The isolation and identification of pulmonary macrophages were discussed and methods for isolating alveolar macrophages in laboratory animals, for obtaining in-situ pulmonary macrophages from experimental animals, for identifying alveolar macrophages, for isolating and identifying blood phagocytes, for isolating monocytes and neutrophilic granulocytes from human blood, for purifying monocytes and polymorphonuclear leukocytes, for purifying blood granulocytes by centrifugal elutriation, for isolating and purifying peritoneal phagocytes, and for isolating and purifying hepatic Kupffer cells from mice were described. The heterogeneity of cellular preparations was discussed. It was noted that in-situ pulmonary macrophages exhibit greater uptake of particles and greater secretion of superoxide anion than free alveolar macrophages. Methods for separating subpopulations of alveolar macrophages by density and for separating dog alveolar macrophages by centrifugal elutriation were summarized. The heterogeneity of peritoneal macrophages and blood neutrophils were also considered.
Laboratory-animals; Lung-cells; Blood-cells; Humans; Phagocytic-activity; Laboratory-techniques; Laboratory-testing; Liver-cells; Biological-material; NIOSH-Author;