Studies were made to determine if exposure of male and female Sprague-Dawley-rats to 25 parts per million (ppm) of 2- methoxyethanol (109864) (2ME) would induce detectable effects in their offspring. Male rats were exposed to 25ppm of 2ME for 7 hours a day for 6 weeks and then, after 2 nonexposure days, were mated with unexposed virgin female rats. Pregnant female rats were exposed to 25ppm of 2ME for 7 hours a day on gestation days 7 to 13 or 14 to 20. Male and female pups from each litter were examined at days 10 through approximately 40 for neuromuscular function, exploratory activity, circadian activity, aversive learning, and appetitive learning. The brains of the newborn pups were subjected to whole brain neurochemical assays, and the brains of 21 day old pups were subjected to assays of the cerebrum, cerebellum, brainstem, and midbrain. No treatment related differences in weight gain were noticed in the offspring. Very few behavioral tests revealed differences from controls: the aversive learning test was the only one exhibiting consistent differences between groups. In contrast, numerous alterations were observed in neurochemical assays between the exposed and control offspring: differences were apparent in acetylcholine, dopamine, norepinephrine, and 5- hydroxytryptamine levels. The authors suggest that inhalation of 25ppm of 2ME during pregnancy can exert a teratogenic effect that is reflected in alterations in the offspring.
NIOSH-Author; Teratogens; Laboratory-animals; Organic-solvents; Reproductive-hazards; Behavior; Neuromotor-system; Sex-factors; Alcohols; Health-hazards;
Author Keywords: Teratology; Reproduction; Reproductive hazard; Industrial solvents; Occupational exposure; Neurochemical deviation; Behavioral teratology; Methoxyethanol