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Stimulation of interleukin-1 production by alveolar macrophages by endotoxin from various bacterial species.

Authors
Lewis-DM
Source
Cotton dust: proceedings of the Ninth Cotton Dust Research Conference, beltwide cotton research conferences, January 9-11, 1985, New Orleans, Louisiana. Wakelyn PJ, Jacobs RR, eds. Memphis, TN: The National Cotton Council of America; 1985 Jan; :157-159
Link
NIOSHTIC No.
00172838
Abstract
The ability of endotoxins from various bacterial species to stimulate production of interleukin-1 (IL1) from alveolar macrophages was studied in-vitro. Samples of purified lipopolysaccharide (LPS) and endotoxin from Escherichia-coli were incubated with alveolar macrophages from Lewis-rats. The cultures were assayed for IL1 using a mouse thymocyte proliferation assay. Dose response curves were constructed. The dose response curves of the LPS and Escherichia-coli endotoxin were very similar, indicating that the LPS moiety in the endotoxin molecule was responsible for the IL1 inducing activity. Purified LPS samples isolated from Enterobacter-agglomerans were also tested. The dose response curve for Enterobacter-agglomerans was shifted to the left of the one for Escherichia-coli. The concentrations of the Enterobacter- agglomerans LPS preparations required to produce half of the maximal induction of IL1 were approximately 1.4 to 8 times lower than that for the Escherichia-coli LPS. The author concludes that LPS derived from Enterobacter-agglomerans is a more potent inducer of IL1 in rat alveolar macrophages than Escherichia-coli LPS. The relative potency of the Enterobacter-agglomerans preparations varies. This may be related to the purity of the preparations.
Keywords
In-vitro-studies; Microbial-test-systems; Microorganisms; Dose-response; Molecular-structure; Bioassays; Immune-reaction; Alveolar-cells
Publication Date
19850101
Document Type
Conference/Symposia Proceedings
Editors
Wakelyn-PJ; Jacobs-RR
Fiscal Year
1985
NTIS Accession No.
NTIS Price
ISBN No.
9780961340810
Source Name
Cotton dust: proceedings of the Ninth Cotton Dust Research Conference, beltwide cotton research conferences, January 9-11, 1985, New Orleans, Louisiana
State
LA; TN
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