Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Cotton bracts tannin is a cytotoxin for porcine endothelial cells.

Authors
Johnson-CM; Hanson-MN; Rohrbach-MS
Source
Cotton dust: proceedings of the Ninth Cotton Dust Research Conference, beltwide cotton research conferences, January 9-11, 1985, New Orleans, Louisiana. Wakelyn PJ, Jacobs RR, eds. Memphis, TN: The National Cotton Council of America; 1985 Jan; :130-133
Link
NIOSHTIC No.
00172830
Abstract
The cytotoxicity of tannin (1401554) isolated from cotton bracts was studied in-vitro. Aqueous extracts of cotton bract tannin and purified tannin were prepared from Acala SJ-2 cotton. These were incubated with chromium-51 loaded porcine endothelial cells from the thoracic aorta and pulmonary arteries for up to 6 hours. Tannin concentrations ranged up to 252 micrograms per milliliter. Cytotoxicity was evaluated by measuring release of chromium-51 from the cells. Dose response curves were constructed. Morphological changes were evaluated by phase contrast and electron microscopy. The cotton bract tannin and purified tannin extracts caused lethal cell injury to both types of cells. The dose response curves were sigmoidal in shape and similar, if not identical, for both the cotton bract and purified tannin bracts. The time dependence of chromium-51 release was biphasic. No chromium-51 was released during the first 3 hours of incubation. Between 3 and 6 hours, chromium-51 was released. This release was linearly related to time. During the first 3 hours, the cells became highly demarcated and swollen. Coincident with the onset of chromium-51 release, the cell nuclei became pycnotic and the cell membranes appeared to collapse upon themselves. The authors conclude that relatively low concentrations of tannin can cause lethal and sublethal injury to endothelial cells. The interaction between tannin and endothelial cells may be a contributing factor in the development of chronic lung disease.
Keywords
NIOSH-Grant; Pulmonary-system-disorders; Natural-products; In-vitro-studies; Cytotoxic-effects; Plant-substances; Histomorphology; Mammalian-cells; Dose-response; Cell-cultures; Mathematical-models
Contact
Internal Medicine Mayo Foundation 200 First Street S W Rochester, MN 55905
CAS No.
1401-55-4;
Publication Date
19850101
Document Type
Conference/Symposia Proceedings
Editors
Wakelyn-PJ; Jacobs-RR
Funding Amount
178383
Funding Type
Grant
Fiscal Year
1985
NTIS Accession No.
NTIS Price
ISBN No.
9780961340810
Identifying No.
Grant-Number-R01-OH-02116
Priority Area
Pulmonary-system-disorders
Source Name
Cotton dust: proceedings of the Ninth Cotton Dust Research Conference, beltwide cotton research conferences, January 9-11, 1985, New Orleans, Louisiana
State
LA; TN; MN
Performing Organization
Mayo Foundation, Rochester, Minnesota
TOP