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Mutagenicity of diesel exhaust particles and oil shale particles dispersed in lecithin surfactant.

Authors
Wallace-WE; Keane-MJ; Hill-CA; Xu-J; Ong-T
Source
J Toxicol Environ Health 1987 May; 21(1-2):163-171
NIOSHTIC No.
00171009
Abstract
The mutagenic activity of pulmonary surfactant (dipalmitoyl- lecithin) dispersed diesel exhaust particulate material from exhaust pipe scrapings, diluted automobile diesel exhaust particulate material collected on filters, and two oil shale ores, were compared to 0.85 percent physiological saline dispersed samples and to dichloromethane extracted samples. Mutagenicity testing was performed by the Ames Salmonella/microsome assay. Responses of the dichloromethane extracted and the dipalmitoyl-lecithin dispersed samples were greater than those observed with saline dispersed samples, although mutagenicity increased with dose in all cases. Addition of S-9 fraction reduced the mutagenicity of all samples, but particularly the dipalmitoyl-lecithin dispersed one. Dispersed rather than dissolved soot fractions were shown to be responsible for mutagenic activity. These results indicate that mutagenic agents (diesel soots) are mutagenic in the Ames Salmonella assay when dispersed in a phospholipid emulsion resembling pulmonary surfactant material. The authors conclude that mutagens associated with inhaled particles may be similarly dispersed into the phospholipid component of pulmonary surfactant in-vivo and effect cellular mutagenesis.
Keywords
NIOSH-Author; Lung-lesions; Lung-tissue; Solvent-extraction; Oil-shale; Toxic-effects; Polycyclic-aromatic-hydrocarbons; Gene-mutation; Particulates; Aqueous-solutions; Solvents; Diesel-exhausts; In-vitro-studies
CODEN
JTEHD6
Publication Date
19870501
Document Type
Journal Article
Fiscal Year
1987
NTIS Accession No.
NTIS Price
Issue of Publication
1-2
ISSN
0098-4108
NIOSH Division
DRDS
Source Name
Journal of Toxicology and Environmental Health
State
WV
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