Particulate diesel exhaust characterization study.
Glaser-R; Cooper-C; Deye-G; Ong-T
Several analyses were made of the diesel exhaust particulates which were used in a study made to determine the effects of long term exposure to coal dust and exhaust from diesel engines on laboratory animals. The diesel engine used was of a type used in underground mining operations, and was equipped with a water scrubber in the exhaust line. Lipid chromatographic fluorescence detection of the solvent extracts of the diesel exhaust components showed fluorescing compounds covering a wide range of polarity to be present in the exhaust samples. Preparative fractionations of solvent extracts of particulate diesel exhaust indicated that the majority of the mass recovered was in the nonpolar fraction. Sufficient quantities of nitroarenes were isolated for identification purposes; high resolution mass spectroscopy with selected ion monitoring was used. 1-Nitropyrene (5522430) and a nitrofluorene isomer were identified in several of the extract fractions. The mutagenic potentials of extracts of diesel particulate exhaust, coal dust and scrubber water and semisolid particulate deposits from the scrubber were determined using the Ames test. Both direct and enzyme activated mutagens were present in the diesel particulate and scrubber water extract and the unextracted scrubber particulate. The authors conclude that the contents of the scrubber may represent a significant health hazard due to the presence of mutagens.
NIOSH-Author; Diesel-exhausts; Diesel-emissions; Coal-dust; Particulate-dust; Nitropyrenes; Air-contamination
NTIS Accession No.
Robert A. Taft Laboratories, NIOSH, U.S. Department of Health and Human Services, 62 pages, 24 references