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In vivo and in vitro evaluations of spermatotoxicity induced by 2-ethoxyethanol treatment.

Authors
Oudiz-D; Zenick-H
Source
Toxicol Appl Pharmacol 1986 Jul; 84(3):576-583
NIOSHTIC No.
00163478
Abstract
The mechanism of 2-ethoxyethanol (110805) (EE) action on male reproductive system was studied in-vivo and in-vitro. Male Long- Evans hooded rats were given 0 or 936 milligram/kilogram of EE by gavage, 5 days/week for 6 weeks. The initial sperm count was 60 million. The count decreased 30 to 40 percent after 5 weeks. By the sixth week, three of ten treated rats were azoospermic and the remaining rats had sperm counts between 5 and 30 million. Test animals showed abnormal sperm morphology, but had about the same motility as the control group. Testes, epididymides, and caudae epididymides weights were lower in the treated animals. The treatment of spermatocytes with ethoxyacetic-acid (627032) (EAA), the reported active metabolite of EE, elicited a significant increase in the lactate:endogeneous ratio and a significant decrease in the 2,4-dinitrophenol:lactate ratio, indicating an enhanced utilization of oxygen by the treated spermatocytes. The simultaneous decrease in the 2,4-dinitrophenol:lactate ratio suggested that spermatocytes were at least partially in an uncoupled oxidative state. The EAA treated cells also showed a large decrease in ATP concentration. The authors concluded the results indicate that EAA interferes with energy metabolism in the pachytene spermatocytes which may partially explain the testicular toxicity.
Keywords
NIOSH-Publication; NIOSH-Grant; Reproductive-system-disorders; In-vivo-studies; In-vitro-studies; Rodents; Testes; Reproductive-hazards; Enzymes; Ethers; Alcohols; Aliphatic-compounds
Contact
Environmental Health University of Cincinnati 3223 Eden Avenue Cincinnati, Ohio 45267
CODEN
TXAPA9
CAS No.
110-80-5; 627-03-2
Publication Date
19860701
Document Type
Journal Article
Funding Amount
218617
Funding Type
Grant
Fiscal Year
1986
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-01272
Issue of Publication
3
ISSN
0041-008X
Priority Area
Reproductive-system-disorders
Source Name
Toxicology and Applied Pharmacology
State
OH
Performing Organization
University of Cincinnati, Cincinnati, Ohio
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