The mutagenicities of 3-chloropropene (107051) (CPP), cyclohexanone (108941), methyl-bromide (74839), N,N-dimethylacetamide (127195) (DMAA), N-N-dimethylformamide (68122) (DMFA), N- methyldicyclohexylamine (7560830) (MDCHA), 2-nitropropane (79469), butylene-oxide (26249207), bis(2-methoxyethyl)ether (111966) (BMEE), hexachloro-1,3-butadiene (87683) (HCB), 2-methoxyethanol (109864) (MOE), styrene-oxide (96093), and 1,1,2,2-tetrachloroethane (79345) (TCE) were studied. Dominant lethal, sperm morphology, and bone marrow cytology assays were performed after inhalation exposure of rats and mice 7 hours per day for 5 days. Unscheduled DNA synthesis was measured in-vitro in human embryonic intestinal cells treated for 3 hours at eight concentrations that ranged as high as 9,900 micrograms per milliliter (microg/ml) and as low as 50microg/ml. Drosophila recessive lethal testing exposed flies to various concentrations of the 13 compounds for varying amounts of time. CPP, cyclohexanone, methyl-bromide, DMAA, DMFA, MDCHA, and 2- nitropropane induced no genetic damage. BMEE and MOE produced a strong antifertility effect and some genetic toxicity, and styrene- oxide produced a similar effect but no genetic toxicity. Butylene- oxide produced negative results except in the bone marrow cytology assay where results were questionable. HCB also produced a questionable bone marrow cytology result and exerted an unclear influence during the dominant lethal assay. TCE produced borderline results during both the bone marrow cytology and sperm morphology assays.
Division of Biomedical and Behavioral Science, NIOSH, U.S. Department of Health and Human Services, Cincinnati, Ohio, 31 pages