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A direct in vivo method for studying the percutaneous absorption of volatile chemicals.

Susten-AS; Dames-B; Niemeier-RW
Proceedings of the 15th Conference on Environmental Toxicology, October 30 - November 1, 1984, Dayton, Ohio. Dayton, OH: Air Force Aerospace Medical Research Laboratory, 1985 May; :322-336
Percutaneous absorption of radiolabeled volatile materials was studied in mice using a skin depot designed to capture the portion of test substance normally lost by evaporation. The skin depot consisted of three components and a guide needle. Hairless-mice were used as the animal model. Metabolism cages used during experiments captured expired breath, urine and feces. The mice were killed at the end of the experiment. The percentage of the dose that evaporated from the skin was measured after desorption of any remaining radioactivity was completed. Skin application sites were digested; volatile labeled materials released during the digestion process were trapped and quantified. Radioactivity was measured for fecal pellets, digested application sites, carcasses, urine, and cage washings. The percent of the applied dose recovered was determined for the skin depot, the guide needle, and the absorbed dose. Experiments with benzene (71432) and toluene (108883) showed that total recovery was above 90 percent. Other tests determined the time required for the skin depot to adsorb the volatilized portion of the dose and if the radioactivity in the depot changed with time. Adsorption onto the sorbent was maximal by 1.5 minutes and remained unchanged for at least 2.5 hours. When the skin depot was tested under simulated experimental conditions for leaks of the volatile test material by migration through the sorbent, skin depots were capable of adsorbing all volatized test material. When in-vivo dermal absorption of benzene and toluene was compared with data obtained in humans and other animals, similar patterns of dermal absorption of the compounds were found from species to species. The author concludes that the experimental model is useful for directly evaluating in-vivo dermal absorption of volatile compounds.
Radiochemical-analysis; Skin-absorption; Industrial-chemicals; Medical-research; Animal-studies; Toxicology; Organic-chemicals; Comparative-toxicology; Analytical-methods; Analytical-models
71-43-2; 108-88-3
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Proceedings of the 15th Conference on Environmental Toxicology, October 30 - November 1, 1984, Dayton, Ohio