Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Induction of murine reaginic antibodies by toluene diisocyanate. An animal model of immediate hypersensitivity reactions to isocyanates.

Authors
Tse-CS; Chen-SE; Bernstein-IL
Source
Am Rev Respir Dis 1979 Oct; 120(4):829-835
NIOSHTIC No.
00136945
Abstract
A murine model was developed for homocytotropic antibodies (HCA) induced by toluene-diisocyanate (26471625) (TDI) in C57B1-mice, DBA- mice, and HRS/J-mice. Protein conjugates were prepared using TDI, human serum albumin (HSA), bovine serum albumin (BSA), or ovalbumin (OVA). Chemical assays were performed to determine the moles of isocyanate bound to protein as a function of reaction time. Mice were intraperitoneally immunized with 100 micrograms (microg) of the various conjugates or carrier proteins mixed with aluminum- hydroxide. Immunologic reactivity of animals immunized with the various carrier proteins was assessed in sera obtained 14 days after primary immunization. The same doses of homologous antigen and aluminum-hydroxide were given 28 to 35 days after the initial injection to determine anamnestic effects. Hapten specificity of HCA was tested by passive cutaneous anaphylactic (PCA) reactions in response to intravenous challenge. Sera from mice immunized with 100microg of unconjugated HSA, BSA, or OVA proteins demonstrated HCA titers of 1 to 40 after challenge. Immunoglobulin-E antibody titers ranging from 1 to 57 to 1 to 113 were obtained when sera from mice immunized with TDI/HSA, TDI/BSA, and TDI/OVA were challenged with respective homologous hapten protein conjugates. Lower PCA titers were obtained when the same sera were challenged with conjugates of TDI coupled with heterologous carriers. There was low cross reacting among conjugates. Carrier and hapten specific effects were demonstrated. The authors conclude that moderately substituted and mono functionally TDI bound proteins are potent immunogens for the production of reaginic antibodies in susceptible strains of mice. The animal model of HCA induced by chemically well characterized protein conjugates of TDI is reproducible.
Keywords
NIOSH-Publication; NIOSH-Grant; Synthetic-rubbers; Analytical-models; Immune-reaction; Animal-studies; Hypersensitivity; Industrial-chemicals; Industrial-medicine; Medical-research
Contact
Internal Medicine University of Cincinnati 231 Bethesda Avenue Cincinnati, Ohio 45267
CODEN
ARDSBL
CAS No.
26471-62-5
Publication Date
19791001
Document Type
Journal Article
Funding Amount
301375
Funding Type
Grant
Fiscal Year
1980
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-00773
Issue of Publication
4
ISSN
0003-0805
Source Name
American Review of Respiratory Disease
State
OH
Performing Organization
University of Cincinnati, Cincinnati, Ohio
TOP