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Drug metabolism in skin. Comparative activity of the mixed-function oxidases, epoxide hydratase, and glutathione S-transferase in liver and skin of the neonatal rat.

Authors
Mukhtar-H; Bickers-DR
Source
Drug Metab Dispos 1981 Jul; 9(4):311-314
NIOSHTIC No.
00136923
Abstract
The activity of aryl-hydrocarbon-hydroxylase (AH) in skin was compared with its activity in other organs following topical application of cutaneous enzyme system inducers. Newborn rats were treated with 100 milligrams per kilogram (mg/kg) skin application of polychlorinated biphenyls (PCB), Aroclor-1254 (11097691), or benz(a)pyrene (50328) (BaP). After 24 hours, animals were killed and skin, liver, lungs, kidneys, intestines, and remaining carcass were prepared for enzyme assay. After topical application of BaP, there was induction of AH in each tissue studied. A greater increase in enzyme activity in skin as compared with other extrahepatic tissues was observed. When total organ activities were compared with respect to the whole body, whole body activity was greater than in liver, which was greater than in skin. After topical application of Aroclor, there were increases in AH activities and 7-ethoxycoumarin deethylase in skin and liver. Glutathione-S-transferase (GSH) activity in neonatal rat liver was induced 40 to 60 percent over controls after application of Aroclor. Cutaneous GSH activity did not increase after skin application of Aroclor. The authors conclude that the topical application of PCB to neonatal rats results in the doubling of hepatic/extrahepatic activities without any detectable effect on the skin enzymes.
Keywords
NIOSH-Publication; NIOSH-Grant; Enzyme-activity; Animal-studies; Skin-exposure; Medical-research; Analytical-methods; Metabolic-study; Biological-effects; Physiological-response; Laboratory-techniques
Contact
Medicine Cleveland V a Hospital 10701 East Blvd Cleveland, Ohio 44106
CODEN
DMDSAI
CAS No.
11097-69-1; 50-32-8
Publication Date
19810701
Document Type
Journal Article
Funding Amount
267904
Funding Type
Grant
Fiscal Year
1981
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-01149
Issue of Publication
4
ISSN
0090-9556
Source Name
Drug Metabolism and Disposition
State
OH
Performing Organization
Case Western Reserve University, Cleveland, Ohio
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